Acta Neuropathologica

, Volume 103, Issue 2, pp 179–187

Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis

  • Akito Ikemoto
  • Shinichi Nakamura
  • Ichiro Akiguchi
  • Asao Hirano
Regular Paper

DOI: 10.1007/s004010100449

Cite this article as:
Ikemoto, A., Nakamura, S., Akiguchi, I. et al. Acta Neuropathol (2002) 103: 179. doi:10.1007/s004010100449

Abstract.

This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.

Amyotrophic lateral sclerosis Presynaptic terminal Spinal cord Synaptic protein

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Akito Ikemoto
    • 1
  • Shinichi Nakamura
    • 2
  • Ichiro Akiguchi
    • 2
  • Asao Hirano
    • 3
  1. 1.Laboratory of Anatomic Pathology, Kyoto University Hospital, 54 Shogoin-Kawaharacho, Sakyoku, Kyoto 606-8507, Japan
  2. 2.Department of Neurology, Faculty of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyoku, Kyoto 606-8507, Japan
  3. 3.Division of Neuropathology, Department of Pathology, Montefiore Medical Center, 111 East 210th Street, Bronx, NY 10467, USA