Acta Neuropathologica

, Volume 96, Issue 2, pp 129–138

Comparison of β-protein/A4 deposits and Alz-50-stained cytoskeletal changes in the hypothalamus and adjoining areas of Alzheimer’s disease patients: amorphic plaques and cytoskeletal changes occur independently

  • J. A. P. van de Nes
  • W. Kamphorst
  • R. Ravid
  • D. F. Swaab
Regular paper

DOI: 10.1007/s004010050872

Cite this article as:
van de Nes, J., Kamphorst, W., Ravid, R. et al. Acta Neuropathol (1998) 96: 129. doi:10.1007/s004010050872

Abstract

Alzheimer’s disease is characterized neuropathologically by senile plaques and cytoskeletal changes. It has been proposed that amorphic plaques would locally induce anterograde propagation of cytoskeletal changes in consecutive neurons followed by amorphic plaque deposition at their axonal terminals. The Alzheimer changes would spread in this way along neural pathways. To test the ‘primary amyloid anatomical cascade hypothesis’, Congo red staining, β-protein/A4 (Aβ) antiserum and Alz-50, which recognizes cytoskeletal changes, were applied to the hypothalamus and adjoining brain areas of five Alzheimer’s disease patients of 40–90 years of age and five age- and sex-matched controls. The results showed that (1) virtually all Aβ plaques in the hypothalamus were of the Congo red-negative amorphic type; (2) amorphic plaques and Alz-50-stained cytokeletal changes were observed not only in all Alzheimer’s disease patients but also in a non-demented, 90-year-old control subject; (3) the density of amorphic plaques in the hypothalamus was unrelated to the duration of the dementia; (4) the density of amorphic plaques was unrelated to that of Alz-50-stained cytoskeletal changes; (5) double-labeling with anti-Aβ and Alz-50 did not show an evident topical relationship between amorphic plaque deposition and the occurrence of cytoskeletal changes; and (6) the distribution of Aβ and Alz-50 staining in five brain areas, for which essential anatomical information is available, did not support the primary amyloid anatomical cascade hypothesis. Amorphic plaques and cytoskeletal changes rather occur independently.

Key words Alzheimer’s disease Alz-50 β-protein Cytoskeletal changes Human hypothalamus 

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. A. P. van de Nes
    • 1
  • W. Kamphorst
    • 3
  • R. Ravid
    • 1
  • D. F. Swaab
    • 1
  1. 1.Netherlands Institute for Brain Research, Amsterdam, The NetherlandsNL
  2. 2.Department of Psychiatry, Valerius Clinic, Free University, Amsterdam, The NetherlandsNL
  3. 3.Department of Neuropathology, Free University, Amsterdam, The NetherlandsNL
  4. 4.Department of Pathology, University Hospital Nijmegen, PO Box 9101, 6500 HB, Nijmegen, The Netherlands Tel.: 31-24-3614314, Fax: 31-24-3540520NL