Acta Neuropathologica

, Volume 91, Issue 1, pp 78–81

Microtubule-associated protein 5 is a component of Lewy bodies and Lewy neurites in the brainstem and forebrain regions affected in Parkinson's disease

  • W. P. Gai
  • P. C. Blumbergs
  • W. W. Blessing
Regular paper

DOI: 10.1007/s004010050395

Cite this article as:
Gai, W., Blumbergs, P. & Blessing, W. Acta Neuropathol (1995) 91: 78. doi:10.1007/s004010050395

Abstract

Ubiquitin-positive Lewy neurites and Lewy bodies are found in idiopathic Parkinson's disease (PD) and diffuse Lewy body disease (DLBD). We found that, in three patients with PD and one with DLBD, microtubule-associated protein 5 (MAP5) immunostaining was consistently present in both Lewy neurites and Lewy bodies throughout the brainstem and forebrain regions affected in the disease. In contrast, other cytoskeletal markers (neurofilaments and MAP2) could be demonstrated in only a small fraction of Lewy bodies and neurites. Confocal microscopy demonstrated that MAP5 immunolabeling was located around the perimeter of the ubiquitin-positive labeling which occupied the central region of the neurite and Lewy body, with some overlap between MAP5 and ubiquitin staining. In contrast, in those Lewy bodies and neurites immunopositive for phosphorylated and non-phosphorylated neurofilament proteins, the neurofilament labeling was quite peripheral to the ubiquitin staining, with little or no overlap. Our results suggest MAP5 is more closely associated with the ubiquitinated proteins of Lewy bodies and neurites than other cytoskeletal proteins.

Key words Lewy neuriteLewy bodyMicrotubule-associated protein 5Parkinson's disease

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • W. P. Gai
    • 1
  • P. C. Blumbergs
    • 2
  • W. W. Blessing
    • 1
  1. 1.Departments of Physiology and Medicine, Centre for Neuroscience, Flinders University, Bedford Park, SA 5042, Australia Tel.: 618-204-4107; Fax: 618-204-5450AU
  2. 2.Department of Neuropathology, Institute of Medical and Veterinary Science, Adelaide, AustraliaAU