Acta Neuropathologica

, Volume 100, Issue 6, pp 673–680

Allograft inflammatory factor-1 defines a distinct subset of infiltrating macrophages/microglial cells in rat and human gliomas

  • M. H. Deininger
  • K. Seid
  • S. Engel
  • R. Meyermann
  • H. J. Schluesener
Regular paper

DOI: 10.1007/s004010000233

Cite this article as:
Deininger, M., Seid, K., Engel, S. et al. Acta Neuropathol (2000) 100: 673. doi:10.1007/s004010000233

Abstract

Allograft inflammatory factor-1 (AIF-1) is a Ca2+-binding peptide that constitutes a potential modulator of macrophage activation and function during the immune response of the brain. Peptides termed microglia response factor-1 or ionized calcium-binding adaptor molecule-1 have been reported to be identical with AIF-1. We have investigated the expression of AIF-1 in the rat C6 glioblastoma and 9L gliosarcoma tumor models and additionally assessed AIF-1 expression in a diverse range of human astrocytomas by immunohistochemistry. AIF-1 was expressed by activated microglial cells and a subset of infiltrating macrophages in areas of infiltrative tumor growth and in compact tumor areas in both rat and human gliomas. Double-labeling experiments in rats and humans characterized the nature and the functional status of AIF-1+ cells. AIF-1 expression was detected in cells expressing major histocompatibility complex class II molecules and in a subset of activated macrophages/microglial cells. All MRP-8+ cells coexpressed AIF-1. In humans, there was a strong correlation of AIF-1-expressing activated macrophages/microglial cells with tumor malignancy (P < 0.0001). These results suggest that AIF-1 defines a distinct subset of tumor-associated activated macrophages/ microglial cells.

Key words Cell cultureCell lineGliomaCalcium-binding proteinsMicroglia enzymologyImmunohistochemistry

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. H. Deininger
    • 1
  • K. Seid
    • 1
  • S. Engel
    • 1
  • R. Meyermann
    • 1
  • H. J. Schluesener
    • 1
  1. 1.Institute of Brain Research, University of Tuebingen, Calwer Strasse 3, 72076 Tuebingen, Germany e-mail: hirnforschung@uni-tuebingen.de, Tel.: +49-7071-2984881, Fax: +49-7071-295456DE