, Volume 126, Issue 4, pp 607-608
Date: 11 Sep 2013

A glioblastoma neurosphere line with alternative lengthening of telomeres

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Neoplastic cells must maintain telomeres in order to replicate extensively, often by inducing telomerase activity. However, some tumors use a telomerase-independent mechanism dependent upon homologous recombination known as alternative lengthening of telomeres (ALT) [1]. ALT has been identified in 11–24 % of glioma tissue samples [24], but has been demonstrated in only one glioma cell line (TG20) [5]. In this brief letter, we describe a second ALT-positive glioblastoma (GBM) cell line.

We examined five stem-like GBM neurosphere lines (HSR-GBM1, 040821, 040622, JHH-GBM10, and JHH-GBM14) by telomere-specific FISH and identified the ultra-bright telomeric DNA foci indicative of ALT [6] in JHH-GBM14 (Fig. 1a), which also contained ALT-associated PML bodies (APBs; inset). These neurospheres were isolated from an untreated primary frontal lobe glioblastoma in a 69-year-old male [7], and examination of the surgical specimen also revealed ALT (Fig. 1b). The percentage of cells displaying ultra ...

C. M. Heaphy and K. C. Schreck equally contributed to this study.