Acta Neuropathologica

, 118:599

Monoclonal antibody specific for IDH1 R132H mutation

  • David Capper
  • Hanswalter Zentgraf
  • Jörg Balss
  • Christian Hartmann
  • Andreas von Deimling
Short Report

DOI: 10.1007/s00401-009-0595-z

Cite this article as:
Capper, D., Zentgraf, H., Balss, J. et al. Acta Neuropathol (2009) 118: 599. doi:10.1007/s00401-009-0595-z

Abstract

IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. We developed a mouse monoclonal antibody targeting the IDH1 R132H mutation. Here, we show the high specificity and sensitivity of this antibody on Western blots and tissue sections from formalin fixed paraffin embedded tumor specimens. This antibody is highly useful for tumor classification, in detecting single infiltrating tumor cells and for the characterization of the cellular role of mutant IDH1 protein.

Keywords

IDH1Monoclonal antibodyImmunohistochemistryAstrocytomaOligodendrogliomaOligoastrocytoma

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • David Capper
    • 1
  • Hanswalter Zentgraf
    • 2
  • Jörg Balss
    • 3
  • Christian Hartmann
    • 1
    • 3
  • Andreas von Deimling
    • 1
    • 3
  1. 1.Department of Neuropathology, Institute of PathologyRuprecht-Karls-University HeidelbergHeidelbergGermany
  2. 2.Monoclonal Antibody UnitGerman Cancer Research CenterHeidelbergGermany
  3. 3.Clinical Cooperation Unit Neuropathology G380German Cancer Research CenterHeidelbergGermany