Colloid and Polymer Science

, Volume 276, Issue 5, pp 388–394

Degradation of hyaluronic acid at the metal surface

  • T. Miyazaki
  • C. Yomota
  • S. Okada
ORIGINAL CONTRIBUTION

DOI: 10.1007/s003960050257

Cite this article as:
Miyazaki, T., Yomota, C. & Okada, S. Colloid Polym Sci (1998) 276: 388. doi:10.1007/s003960050257

Abstract

 When the viscosity of hyaluronic acid (HA) solution was measured with a rotational visco-meter, depolymerization occurred only by leaving the sample in the cup of the viscometer, while the solution in a glass test tube at the same temperature, 37 °C, was practically stable during the entire period of investigation. As the parts where the sample solution had contact with the viscometer are stainless steel, the participation of metal ions in the depolymerization was suspected. Therefore, experiments were carried out with stainless-steel beads in the HA solution. The results show that the molecular weight of HA decreased on exposure to the beads under aerobic conditions. The addition of 10 beads to 2 ml of a 0.1% HA solution prepared with 0.2 M NaCl reduced the molecular weight to about 75% of the initial value after 4 h. The degradation rate was proportional to the surface area of the beads. However, no dissolution of metal ion was detected, and therefore, this reaction was thought to proceed at the surface of the metal.

Key words Hyaluronic acid degradation molecular weight metal surface hydroxyl radical 

Copyright information

© Steinkopff Verlag 1998

Authors and Affiliations

  • T. Miyazaki
    • 1
  • C. Yomota
    • 1
  • S. Okada
    • 1
  1. 1.National Institute of Health Sciences, Osaka 1-1-43 Hoenzaka Chuo-ku, Osaka 540 JapanJP

Personalised recommendations