Colloid and Polymer Science

, Volume 289, Issue 3, pp 269–280

Synthesis and multidisciplinary characterization of polyelectrolyte multilayer-coated nanogold with improved stability toward aggregation

  • Subhra Mandal
  • Alois Bonifacio
  • Francesco Zanuttin
  • Valter Sergo
  • Silke Krol
Original Contribution

DOI: 10.1007/s00396-010-2343-2

Cite this article as:
Mandal, S., Bonifacio, A., Zanuttin, F. et al. Colloid Polym Sci (2011) 289: 269. doi:10.1007/s00396-010-2343-2

Abstract

An interesting nanodrug delivery system is polyelectrolyte multilayer-coated nanogold. For better understanding of the binding of polycations or the counter-indicative deposition of polyanions on the citrate-stabilized gold nanoparticles, we used a surface-enhanced Raman spectroscopy to characterize the orientation of the polyions towards the gold surface. It was found that poly-allylamine replaces citrate molecules while the polyanion, poly-styrene sulfonate, intercalates in the citrate shell. One of the major obstacles for polyelectrolyte-coated nanogold is its tendency to agglomerate in the presence of high ion concentration as present, e.g., in blood. A novel encapsulation protocol for polyelectrolyte multilayer coating of gold nanoparticles was developed to successfully overcome this drawback. Moreover, electrostatic functionalization of the polyelectrolyte shell with a model target molecule for cancer, folic acid, induced a significant increase in the particle uptake in folate-receptor over-expressing breast cancer cell lines, VP 229 and MDA MB 231, compared to non-targeted particles or cells (non-activated macrophages) not expressing the folate receptor.

Keywords

Gold nanoparticlesLayer-by-layerPolyelectrolyteSERSTargeted deliveryCancer

Supplementary material

396_2010_2343_MOESM1_ESM.doc (120 kb)
ESM 1(DOC 119 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Subhra Mandal
    • 1
  • Alois Bonifacio
    • 2
  • Francesco Zanuttin
    • 3
  • Valter Sergo
    • 2
  • Silke Krol
    • 3
    • 4
  1. 1.SISSA—International School for Advanced StudiesTriesteItaly
  2. 2.Centre of Excellence for Nanostructured MaterialsUniversity of TriesteTriesteItaly
  3. 3.NanoBioMed lab @ LANADA, CBM S.c.r.l—Cluster in BiomedicineTriesteItaly
  4. 4.European Centre for NanomedicineFondazione I.R.C.C.S. Istituto Neurologico “Carlo Besta”MilanItaly