Colloid and Polymer Science

, Volume 284, Issue 5, pp 520–528

Effects of l-arginine on aggregates of fatty-acid/potassium soap in the aqueous media

  • Ayako Hirai
  • Hideya Kawasaki
  • Shimon Tanaka
  • Norio Nemoto
  • Masao Suzuki
  • Hiroshi Maeda
Original contribution

DOI: 10.1007/s00396-005-1423-1

Cite this article as:
Hirai, A., Kawasaki, H., Tanaka, S. et al. Colloid Polym Sci (2006) 284: 520. doi:10.1007/s00396-005-1423-1

Abstract

In the present study, we investigated the effects of l-arginine on aggregates of fatty acid/fatty soap in the aqueous media as a function of pH, by means of hydrogen ion titration, viscoelastic measurement, cryo-transmission electron microscopy and phase contrast microscopy. We found out that l-arginine effectively inhibits the oil droplet growth of oleic acid or octanoic acid. The effect is explained in terms of the adsorption of arginine at the microscopic drop surface, or at the oil/water microinterface through the hydrophobic effect assisted by the hydrogen bonds between carboxyl group of fatty acid and carboxylate of arginine. As to the crystallization of lauric acid at temperatures below the melting point of the hydrocarbon chain, arginine is not effective. In addition, we also found out that the strong binding of arginine cation to anionic oleate micells induces the dominant micellar growth. l-arginine has been used in many refolding systems to suppress protein aggregation. These effects of l-arginine on the aggregates of fatty acid/fatty soap in the aqueous media observed in the present study is expected to form a basis to the specific function displayed in the protein refolding.

Keywords

l-arginine Arginine oleate Arginine octanoate Vesicle Acid-soap 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Ayako Hirai
    • 1
  • Hideya Kawasaki
    • 1
  • Shimon Tanaka
    • 1
  • Norio Nemoto
    • 2
  • Masao Suzuki
    • 3
  • Hiroshi Maeda
    • 1
  1. 1.Division of Chemistry, Faculty of ScienceKyushu UniversityFukuokaJapan
  2. 2.Department of Molecular and Material Sciences, IGSESKyushu UniversityFukuokaJapan
  3. 3.Research Institute of Biological Materials Japan, Ltd.SeikaJapan

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