Basic Research in Cardiology

, 103:537

Effects of unipolar stimulation on voltage and calcium distributions in the isolated rabbit heart

  • Veniamin Y. Sidorov
  • Mark R. Holcomb
  • Marcella C. Woods
  • Richard A. Gray
  • John P. Wikswo
ORIGINAL CONTRIBUTION

DOI: 10.1007/s00395-008-0740-1

Cite this article as:
Sidorov, V.Y., Holcomb, M.R., Woods, M.C. et al. Basic Res Cardiol (2008) 103: 537. doi:10.1007/s00395-008-0740-1

Abstract

Background

The effect of electric stimulation on the polarization of cardiac tissue (virtual electrode effect) is well known; the corresponding response of intracellular calcium concentration ([Ca2+]i) and its dependence on coupling interval between conditioning stimulus (S1) and test stimulus (S2) has yet to be elucidated.

Objective

Because uncovering the transmembrane potential (Vm)–[Ca2+]i relationship during an electric shock is imperative for understanding arrhythmia induction and defibrillation, we aimed to study simultaneous Vm and [Ca2+]i responses to strong unipolar stimulation.

Methods

We used a dual-camera optical system to image concurrently Vm and [Ca2+]i responses to unipolar stimulation (20 ms ± 20 mA) in Langendorff-perfused rabbit hearts. RH-237 and Rhod-2 fluorescent dyes were used to measure Vm and [Ca2+]i, respectively. The S1–S2 interval ranged from 10 to 170 ms to examine stimulation during the action potential.

Results

The [Ca2+]i deflections were less pronounced than changes in Vm for all S1–S2 intervals. For cathodal stimulation, [Ca2+]i at the central virtual cathode region increased with prolongation of S1–S2 interval. For anodal stimulation, [Ca2+]i at the central virtual anode area decreased with shortening of the S1–S2 interval. At very short S1–S2 intervals (10–20 ms), when S2 polarization was superimposed on the S1 action potential upstroke, the [Ca2+]i distribution did not follow Vm and produced a more complex pattern. After S2 termination [Ca2+]i exhibited three outcomes in a manner similar to Vm: non-propagating response, break stimulation, and make stimulation.

Conclusions

Changes in the [Ca2+]i distribution correlate with the behavior of the Vm distribution for S1–S2 coupling intervals longer than 20 ms; at shorter intervals S2 creates more heterogeneous [Ca2+]i distribution in comparison with Vm. Stimulation in diastole and at very short coupling intervals caused Vm–[Ca2+]i uncoupling at the regions of positive polarization (virtual cathode).

Keywords

calcium dynamicscardiac electrophysiologymembrane potentialoptical mapping

Abbreviations

[Ca2+]i

intracellular calcium concentration

Vm

transmembrane potential

AP

action potential

CT

calcium transient

S1

conditioning stimulus

S2

testing stimulus

SR

sarcoplasmic reticulum

VT

ventricular tachycardia

VF

ventricular fibrillation

VC

virtual cathode

VA

virtual anode

NCX

Na+/Ca2+ exchanger

ICaL

current via the L-type Ca2+ channels

INa/Ca

current via Na+/Ca2+ exchanger

Copyright information

© Springer 2008

Authors and Affiliations

  • Veniamin Y. Sidorov
    • 1
    • 2
    • 3
  • Mark R. Holcomb
    • 2
  • Marcella C. Woods
    • 1
  • Richard A. Gray
    • 1
    • 3
    • 5
  • John P. Wikswo
    • 1
    • 2
    • 3
    • 4
  1. 1.Dept. of Biomedical EngineeringVanderbilt UniversityNashvilleUSA
  2. 2.Dept. of Physics and AstronomyVanderbilt UniversityNashvilleUSA
  3. 3.Vanderbilt Institute for Integrative, Biosystems Research and EducationVanderbilt UniversityNashvilleUSA
  4. 4.Dept. of Molecular Physiology and BiophysicsVanderbilt UniversityNashvilleUSA
  5. 5.Dept. of Biomedical EngineeringUniversity of AlabamaBirminghamUSA