European Journal of Nutrition

, Volume 39, Issue 5, pp 207–212

Effect of complex polyphenols and tannins from red wine on DNA oxidative damage of rat colon mucosa in vivo

Authors

  • L. Giovannelli
    • Dept. of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, 50 139 Italy, e-mail: lisag@server1.pharm.unifi.it
  • G. Testa
    • Dept. of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, 50 139 Italy, e-mail: lisag@server1.pharm.unifi.it
  • C. De Filippo
    • Dept. of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, 50 139 Italy, e-mail: lisag@server1.pharm.unifi.it
  • V. Cheynier
    • Research Unit Biopolymers and Aromas INRA-ISVV, Montpellier, France
  • M.N. Clifford
    • School of Biological Sciences, University of Surrey, Guildford, U. K.
  • P. Dolara
    • Dept. of Pharmacology, University of Florence, Viale Pieraccini 6, Florence, 50 139 Italy, e-mail: lisag@server1.pharm.unifi.it
Original contribution

DOI: 10.1007/s003940070013

Cite this article as:
Giovannelli, L., Testa, G., De Filippo, C. et al. Eur J Nutr (2000) 39: 207. doi:10.1007/s003940070013

Summary

Background: Dietary polyphenols have been reported to have a variety of biological actions, including anti-carcinogenic, antioxidant and anti-inflammatory activities.

Aim of the study: In the present study we have evaluated the effect of an oral treatment with complex polyphenols and tannins from red wine and tea on DNA oxidative dammage in the rat colon mucosa.

Methods: Isolated colonocytes were prepared from the colon mucosa of rats treated for ten days with either wine complex polyphenols (57.2 mg/kg/d) or thearubigin (40 mg/kg/d) by oral gavage. Colonocyte oxidative DNA damage was analysed at the single cell level using a modification of the comet assay technique.

Results: These results show that wine complex polyphenols and tannins induce a significant decrease (−62% for pyrimidine and −57% for purine oxidation) in basal DNA oxidative damage in colon mucosal cells without affecting the basal level of single-strand breaks. On the other hand, tea polyphenols, namely a crude extract of thearubigin, did not affect either strand breaks or pyrimidine oxidation in colon mucosal cells.

Conclusions Our experiments are the first demonstration that dietary polyphenols can modulate in vivo oxidative damage in the gastrointestinal tract of rodents. These data support the hypothesis that dietary polyphenols might have both a protective and a therapeutic potential in oxidative damage-related pathologies.

Key words Antioxidant – oxidative stress – flavonoids – comet assay – isolated colonocytes

Copyright information

© Steinkopff Verlag 2000