European Journal of Nutrition

, Volume 39, Issue 5, pp 194–200

Expression of glucose transporters in lactating human mammary gland epithelial cells

  • S. Obermeier
  • B. Hüselweh
  • H. Tinel
  • R.H.K. Kinne
  • C. Kunz
Original contribution

DOI: 10.1007/s003940070011

Cite this article as:
Obermeier, S., Hüselweh, B., Tinel, H. et al. Eur J Nutr (2000) 39: 194. doi:10.1007/s003940070011

Summary

Background. Human milk contains 60–80 g/l lactose and and oligosaccharides. To synthesize this large amount of carbohydrates, the lacting mammary gland has a high demand for precursor molecules. such as glucose and galactose. Aim of the study. In the present study we investigated the molecular basis for the uptake of glucose and galactose into the human mammary gland. Methods. Using RT-PCR, Southern and Western blotting we analyzed the expression of SGLT1 (sodium glocose cotransporter 1) and GLUT1 (sodium independent glucose transporter) in epithelial cells isolated from fresh human milk. Results. Southern blot analysis of the amplications revealed the expression of SGLT1 mRNA but not of GLUT1 mRNA in milk epithelial cells. Using Western blotting, SGLT1 protein was identified in human milk cells. Conclusions. Our findings indicate that 1) the cell fraction isolated from fresh human milk is a suitable model for investigating gene expression in the human mammary gland and 2) lactating human mammary gland epithelial cells are supplied with monosaccharides mainly via SGLT1.

Key words SGLT1 – GLUT1 – lactation – human milk
List of abbreviations SGLT, sodium dependent glucose transporter; GLUT, sodium independent glucose transporter; RT, reverse transcription; DIG, digoxigenin-->

Copyright information

© Steinkopff Verlag 2000

Authors and Affiliations

  • S. Obermeier
    • 1
  • B. Hüselweh
    • 2
  • H. Tinel
    • 2
  • R.H.K. Kinne
    • 2
  • C. Kunz
    • 1
  1. 1.Forschungsinstitut für Kinderernährung, Heinstück 11, 45225 Dortmund, GermanyDE
  2. 2.Max-Planck-Institut fpr molekulare Physiologie, Otto-Hahn-Str. 11, 45227 Dortmund, GermanyDE
  3. 3.Universität Giessen, Wilhelmstrasse 20, 35392 Gießen, Germany, e-mail: Clemens.Kunz@ernaehrung.uni-giessen.deDE