European Journal of Nutrition

, Volume 53, Issue 3, pp 929–937

High dietary taurine inhibits myocardial apoptosis during an atherogenic diet: association with increased myocardial HSP70 and HSF-1 but not caspase 3

Authors

  • M. Setyarani
    • Department of Medicine, Austin HealthUniversity of Melbourne
  • A. Zinellu
    • Department of Biomedical SciencesUniversity of Sassari
  • C. Carru
    • Department of Biomedical SciencesUniversity of Sassari
    • College of Health and BiomedicineVictoria University
Original Contribution

DOI: 10.1007/s00394-013-0596-5

Cite this article as:
Setyarani, M., Zinellu, A., Carru, C. et al. Eur J Nutr (2014) 53: 929. doi:10.1007/s00394-013-0596-5

Abstract

Background and aim

Apoptosis is a major cause of myocyte death, and taurine is anti-apoptotic. Heat shock protein 70 (HSP70) (which is regulated by heat shock factor—HSF-1) is also anti-apoptotic, and caspase 3 stimulates the apoptotic pathway. This study investigated whether taurine affects atherogenic diet-induced myocardial apoptosis, and whether HSP70, HSF-1 and caspase 3 are involved.

Methods

New Zealand white rabbits were divided into 3 groups for 4 weeks according to their diet. Group 1 (control) was fed a normal rabbit diet; Group 2 (MC) received a normal rabbit diet with 1 % methionine plus 0.5 % cholesterol. Group 3 received MC diet + 2.5 % taurine (MCT).

Results

The atherogenic diet did not affect myocardial HSP70 or HSF-1 protein, but increased myocardial apoptotic nuclei to 40 % (p < 0.01) versus 7 % in con and 12 % in MCT (p < 0.01). However, in MCT, myocardial HSP70 expression increased by 42.7 % versus con and MC (p = 0.016), HSF-1 by 12 % versus con and MC (p < 0.05), and total nuclei count increased by 37 % versus MC (p < 0.05). Caspase 3 subunits remained unchanged in all groups, and HSP70 was increased approximately twofold in endothelial layer of arterioles (p = 0.01).

Conclusion

This study shows that taurine could reduce myocardial apoptotic nuclei and thus confer myocardial cytoprotection via stimulating myocardial HSP70 via HSF-1 and caspase 3-independent mechanisms.

Keywords

CholesterolHomocysteineMethionineTaurineHypochlorite

Copyright information

© Springer-Verlag Berlin Heidelberg 2013