European Journal of Nutrition

, Volume 51, Issue 6, pp 707–718

Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function

Authors

    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Douglas Popp Marin
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Anaysa Paola Bolin
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Rita de Cássia Santos Macedo
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Thais Regina Campoio
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Claudio FinetoJr.
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • Beatriz Alves Guerra
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
  • José Roberto Leite
    • Departamento de PsicobiologiaUniversidade Federal de São Paulo UNIFESP
  • Marcelo Paes Barros
    • Postgraduate Program, Health Sciences, CBSCruzeiro do Sul University
    • Postgraduate Program, Human Movement Sciences Institute of Physical Activity and Sport SciencesCruzeiro do Sul University
  • Rita Mattei
    • Departamento de PsicobiologiaUniversidade Federal de São Paulo UNIFESP
Original Contribution

DOI: 10.1007/s00394-011-0250-z

Cite this article as:
Otton, R., Marin, D.P., Bolin, A.P. et al. Eur J Nutr (2012) 51: 707. doi:10.1007/s00394-011-0250-z

Abstract

Purpose

Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a “good nutritional intervention” with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. The present study was designed to investigate the effect of daily fish oil (FO—10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA—1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes.

Methods

This study was conducted by measurements of lymphocyte proliferation capacity, ROS production [superoxide (O2•−) and hydrogen peroxide (H2O2)], nitric oxide (NO) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release.

Results

After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O2•−, H2O2 and NO production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. l-selectin was increased by FO, and IL-1β was decreased only by ASTA supplementation.

Conclusion

We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO.

Keywords

AntioxidantAstaxanthinCarotenoidFish oilLeukocytesLymphocyteOxidative stressn-3 fatty acids

Copyright information

© Springer-Verlag 2011