European Journal of Nutrition

, Volume 51, Issue 3, pp 335–342

Differential effects of 1α,25-dihydroxycholecalciferol on MCP-1 and adiponectin production in human white adipocytes

Authors

    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Anna Eriksson
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Thomas Dunlop
    • Department of PhysiologyInstitute of Biomedicine, Faculty of Medicine, Biomedicum Helsinki
  • Niklas Mejhert
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Ingrid Dahlman
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Gaby Åström
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Eva Sjölin
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Kerstin Wåhlén
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Carsten Carlberg
    • Department of BiosciencesUniversity of Eastern Finland
  • Jurga Laurencikiene
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Per Hedén
    • Department of Plastic SurgeryAkademikliniken
  • Peter Arner
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
  • Mikael Rydén
    • Department of Medicine HuddingeLipid Laboratory, Karolinska Institutet, NVS, Karolinska University Hospital Huddinge
Original Contribution

DOI: 10.1007/s00394-011-0218-z

Cite this article as:
Lorente-Cebrián, S., Eriksson, A., Dunlop, T. et al. Eur J Nutr (2012) 51: 335. doi:10.1007/s00394-011-0218-z

Abstract

Background/aim

Obesity is characterized by a low-grade inflammation in white adipose tissue (WAT), which promotes insulin resistance. Low serum levels of 1α,25-dihydroxycholecalciferol (DHCC) associate with insulin resistance and higher body mass index although it is unclear whether vitamin D supplementation improves insulin sensitivity. We investigated the effects of DHCC on adipokine gene expression and secretion in adipocytes focusing on two key factors with pro-inflammatory [monocyte chemoattractant protein-1 (MCP-1/CCL2)] and anti-inflammatory [adiponectin (ADIPOQ)] effects.

Methods

Pre-adipocytes were isolated from human subcutaneous WAT and cultured until full differentiation. Differentiated adipocytes were either pre-treated with DHCC (10−7 M) and subsequently incubated with tumor necrosis factor-α (TNFα, 100 ng/mL) or concomitantly incubated with TNFα/DHCC. MCP1 and adiponectin mRNA expression was measured by RT–PCR and protein release by ELISA.

Results

DHCC was not toxic and did not affect adipocyte morphology or the mRNA levels of adipocyte-specific genes. TNFα induced a significant increase in CCL2 mRNA and protein secretion, while DHCC alone reduced CCL2 mRNA expression (~25%, p < 0.05). DHCC attenuated TNFα-induced CCL2 mRNA expression in both pre-incubation (~15%, p < 0.05) and concomitant (~60%, p < 0.01) treatments. TNFα reduced ADIPOQ mRNA (~80%) and secretion (~35%). DHCC alone decreased adiponectin secretion to a similar degree (~35%, p < 0.05). Concomitant treatment with DHCC/TNFα for 48 h had an additive effect, resulting in a pronounced reduction in adiponectin secretion (~70%).

Conclusions

DHCC attenuates MCP-1 and adiponectin production in human adipocytes, thereby reducing the expression of both pro- and anti-inflammatory factors. These effects may explain the difficulties so far in determining the role of DHCC in insulin sensitivity and obesity in humans.

Keywords

Adipocyte1α,25-dihydroxycholecalciferolMCP-1AdiponectinInsulin resistanceObesity

Copyright information

© Springer-Verlag 2011