European Journal of Nutrition

, Volume 51, Issue 1, pp 69–79

The effect of acute pre-exercise dark chocolate consumption on plasma antioxidant status, oxidative stress and immunoendocrine responses to prolonged exercise

  • Glen Davison
  • Robin Callister
  • Gary Williamson
  • Karen A. Cooper
  • Michael Gleeson
Original Contribution

DOI: 10.1007/s00394-011-0193-4

Cite this article as:
Davison, G., Callister, R., Williamson, G. et al. Eur J Nutr (2012) 51: 69. doi:10.1007/s00394-011-0193-4

Abstract

Purpose

Acute antioxidant supplementation may modulate oxidative stress and some immune perturbations that typically occur following prolonged exercise. The aims of the present study were to examine the effects of acutely consuming dark chocolate (high polyphenol content) on plasma antioxidant capacity, markers of oxidative stress and immunoendocrine responses to prolonged exercise.

Methods

Fourteen healthy men cycled for 2.5 h at ~60% maximal oxygen uptake 2 h after consuming 100 g dark chocolate (DC), an isomacronutrient control bar (CC) or neither (BL) in a randomised-counterbalanced design.

Results

DC enhanced pre-exercise antioxidant status (P = 0.003) and reduced by trend (P = 0.088) 1 h post-exercise plasma free [F2-isoprostane] compared with CC (also, [F2-isoprostane] increased post-exercise in CC and BL but not DC trials). Plasma insulin concentration was significantly higher pre-exercise (P = 0.012) and 1 h post-exercise (P = 0.026) in the DC compared with the CC trial. There was a better maintenance of plasma glucose concentration on the DC trial (2-way ANOVA trial × time interaction P = 0.001), which decreased post-exercise in all trials but was significantly higher 1 h post-exercise (P = 0.039) in the DC trial. There were no between trial differences in the temporal responses (trial × time interactions all P > 0.05) of hypothalamic–pituitary–adrenal axis stress hormones, plasma interleukin-6, the magnitude of leukocytosis and neutrophilia and changes in neutrophil function.

Conclusion

Acute DC consumption may affect insulin, glucose, antioxidant status and oxidative stress responses, but has minimal effects on immunoendocrine responses, to prolonged exercise.

Keywords

PolyphenolsDark chocolateExerciseNeutrophilOxidative stressInnate immune system

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Glen Davison
    • 2
  • Robin Callister
    • 5
  • Gary Williamson
    • 3
    • 4
  • Karen A. Cooper
    • 3
  • Michael Gleeson
    • 1
  1. 1.School of Sport, Exercise and Health SciencesLoughborough UniversityLoughboroughUK
  2. 2.Department of Sport and Exercise ScienceAberystwyth UniversityCeredigion, AberystwythUK
  3. 3.Nestle Research CenterLausanne 26Switzerland
  4. 4.School of Food Science and NutritionUniversity of LeedsLeedsUK
  5. 5.School of Biomedical Sciences and PharmacyUniversity of NewcastleNewcastleAustralia