European Journal of Nutrition

, Volume 49, Issue 8, pp 447–457

Astaxanthin addition improves human neutrophils function: in vitro study

  • Rita C. Macedo
  • Anaysa P. Bolin
  • Douglas P. Marin
  • Rosemari Otton
Original Contribution

DOI: 10.1007/s00394-010-0103-1

Cite this article as:
Macedo, R.C., Bolin, A.P., Marin, D.P. et al. Eur J Nutr (2010) 49: 447. doi:10.1007/s00394-010-0103-1

Abstract

Purpose

The aim of the present study was to evaluate the in vitro effect of carotenoid astaxanthin (ASTA) on the phagocytic and microbicidal capacities, cytokine release, and reactive oxygen species production in human neutrophils.

Methods

The following parameters were evaluated: cytotoxic effect of ASTA on human neutrophils viability, phagocytic and microbicidal capacities of neutrophils by using Candida albicans assay, intracellular calcium mobilization (Fura 2-AM fluorescent probe), superoxide anion (lucigenin and DHE probes), hydrogen peroxide (H2O2, phenol red), and nitric oxide (NO·) (Griess reagent) production, activities of antioxidant enzymes (total/Mn-SOD, CAT, GPx, and GR), oxidative damages in biomolecules (TBARS assay and carbonyl groups), and cytokine (IL-6 and TNF-alpha) release.

Results

Astaxanthin significantly improves neutrophil phagocytic and microbicidal capacity, and increases the intracellular calcium concentration and NO· production. Both functional parameters were accompanied by a decrease in superoxide anion and hydrogen peroxide and IL-6 and TNF-α production. Oxidative damages in lipids and proteins were significantly decreased after ASTA-treatment.

Conclusions

Taken together our results are supportive to a beneficial effect of astaxanthin-treatment on human neutrophils function as demonstrated by increased phagocytic and fungicide capacity as well as by the reduced superoxide anion and hydrogen peroxide production, however, without affecting neutrophils capacity to kill C. albicans. This process appears to be mediated by calcium released from intracellular storages as well as nitric oxide production.

Keywords

Antioxidant Astaxanthin Carotenoids Leukocytes Neutrophil Oxidative stress 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Rita C. Macedo
    • 1
  • Anaysa P. Bolin
    • 1
  • Douglas P. Marin
    • 1
  • Rosemari Otton
    • 1
    • 2
  1. 1.Postgraduate Program, Health Science, CBSCruzeiro do Sul UniversitySão PauloBrazil
  2. 2.Postgraduate Program, Human Movement Sciences Institute of Physical Activity and Sport Sciences, ICAFECruzeiro do Sul UniversitySão PauloBrazil

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