European Journal of Nutrition

, Volume 49, Issue 6, pp 345–355

Bilberry juice modulates plasma concentration of NF-κB related inflammatory markers in subjects at increased risk of CVD

Authors

  • Anette Karlsen
    • Department of Nutrition, Institute of Basic Medical SciencesUniversity of Oslo
  • Ingvild Paur
    • Department of Nutrition, Institute of Basic Medical SciencesUniversity of Oslo
  • Siv K. Bøhn
    • Department of Nutrition, Institute of Basic Medical SciencesUniversity of Oslo
  • Amrit K. Sakhi
    • Department of Nutrition, Institute of Basic Medical SciencesUniversity of Oslo
  • Grethe I. Borge
    • Nofima Mat AS
  • Mauro Serafini
    • Antioxidant Research Laboratory, Unit of Human NutritionINRAN
  • Iris Erlund
    • Department of Chronic Disease PreventionNational Institute for Health and Welfare
  • Petter Laake
    • Department of Biostatistics, Institute of Basic Medical SciencesUniversity of Oslo
  • Serena Tonstad
    • Department of Preventive CardiologyUllevaal University Hospital
    • Department of Nutrition, Institute of Basic Medical SciencesUniversity of Oslo
Original Contribution

DOI: 10.1007/s00394-010-0092-0

Cite this article as:
Karlsen, A., Paur, I., Bøhn, S.K. et al. Eur J Nutr (2010) 49: 345. doi:10.1007/s00394-010-0092-0

Abstract

Purpose

Bilberries are abundant in polyphenols. Dietary polyphenols have been associated with strategies for prevention and treatment of chronic inflammatory diseases. We investigated the effect of bilberry juice on serum and plasma biomarkers of inflammation and antioxidant status in subjects with elevated levels of at least one risk factor for cardiovascular disease (CVD).

Methods

In a randomized controlled trial, participants consumed either bilberry juice (n = 31) or water (n = 31) for 4 weeks.

Results

Supplementation with bilberry juice resulted in significant decreases in plasma concentrations of C-reactive protein (CRP), interleukin (IL)-6, IL-15, and monokine induced by INF-γ (MIG). Unexpectedly, an increase in the plasma concentration of tumor nuclear factor-α (TNF-α) was observed in the bilberry group. CRP, IL-6, IL15, MIG, and TNF-α are all target genes of nuclear factor- kappa B (NF-κB), —a transcription factor that is crucial in orchestrating inflammatory responses. Plasma quercetin and p-coumaric acid increased in the bilberry group, otherwise no differences were observed for clinical parameters, oxidative stress or antioxidant status. Furthermore, we studied the effect of polyphenols from bilberries on lipopolysaccharide (LPS)-induced NF-κB activation in a monocytic cell line. We observed that quercetin, epicatechin, and resveratrol inhibited NF-κB activation.

Conclusions

These findings suggest that supplementation with bilberry polyphenols may modulate the inflammation processes. Further testing of bilberry supplementation as a potential strategy in prevention and treatment of chronic inflammatory diseases is warranted.

Keywords

BilberryNF-κBCytokinesPolyphenolsHuman interventionCell culture

Abbreviations

CVD

Cardiovascular disease

CRP

C-reactive protein

IL

Interleukin

MIG

Monokine induced by INF-γ

RANTES

Regulated upon activation, normal T cell expressed and secreted

TNF-α

Tumor nuclear factor-α

NF-κB

Nuclear factor-kappa B

LPS

Lipopolysaccharide

BP

Blood pressure

LDL

Low-density lipoprotein

HDL

High density lipoprotein

SRM

Standardized reference materials

FRAP

Ferric reducing/antioxidant power

TRAP

Total peroxyl-radical trapping activity

TE

Trolox equivalents

ORAC

Oxygen radical absorbance capacity

PCA

Perchloric acid

AA

Ascorbic acid

DHAA

Dehydroascorbic acid

TAA

Total ascorbic acid

HPLC

High performance liquid chromatography

GSH

Glutathione

D-ROM

Diacrons reactive oxygen metabolites

WBC

White blood cells

γ-GT

γ-glutamyltransferase

Supplementary material

394_2010_92_MOESM1_ESM.doc (70 kb)
Supplementary material 1 (DOC 70 kb)

Copyright information

© Springer-Verlag 2010