Bilberry juice modulates plasma concentration of NF-κB related inflammatory markers in subjects at increased risk of CVD
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- Karlsen, A., Paur, I., Bøhn, S.K. et al. Eur J Nutr (2010) 49: 345. doi:10.1007/s00394-010-0092-0
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Bilberries are abundant in polyphenols. Dietary polyphenols have been associated with strategies for prevention and treatment of chronic inflammatory diseases. We investigated the effect of bilberry juice on serum and plasma biomarkers of inflammation and antioxidant status in subjects with elevated levels of at least one risk factor for cardiovascular disease (CVD).
In a randomized controlled trial, participants consumed either bilberry juice (n = 31) or water (n = 31) for 4 weeks.
Supplementation with bilberry juice resulted in significant decreases in plasma concentrations of C-reactive protein (CRP), interleukin (IL)-6, IL-15, and monokine induced by INF-γ (MIG). Unexpectedly, an increase in the plasma concentration of tumor nuclear factor-α (TNF-α) was observed in the bilberry group. CRP, IL-6, IL15, MIG, and TNF-α are all target genes of nuclear factor- kappa B (NF-κB), —a transcription factor that is crucial in orchestrating inflammatory responses. Plasma quercetin and p-coumaric acid increased in the bilberry group, otherwise no differences were observed for clinical parameters, oxidative stress or antioxidant status. Furthermore, we studied the effect of polyphenols from bilberries on lipopolysaccharide (LPS)-induced NF-κB activation in a monocytic cell line. We observed that quercetin, epicatechin, and resveratrol inhibited NF-κB activation.
These findings suggest that supplementation with bilberry polyphenols may modulate the inflammation processes. Further testing of bilberry supplementation as a potential strategy in prevention and treatment of chronic inflammatory diseases is warranted.
KeywordsBilberry NF-κB Cytokines Polyphenols Human intervention Cell culture
Monokine induced by INF-γ
Regulated upon activation, normal T cell expressed and secreted
Tumor nuclear factor-α
Nuclear factor-kappa B
High density lipoprotein
Standardized reference materials
Ferric reducing/antioxidant power
Total peroxyl-radical trapping activity
Oxygen radical absorbance capacity
Total ascorbic acid
High performance liquid chromatography
Diacrons reactive oxygen metabolites
White blood cells