European Journal of Nutrition

, Volume 49, Issue 2, pp 101–109

Naringenin enhances insulin-stimulated tyrosine phosphorylation and improves the cellular actions of insulin in a dietary model of metabolic syndrome

Authors

  • Sriramajayam Kannappan
    • Department of Biochemistry and Biotechnology, Faculty of ScienceAnnamalai University
    • Department of Biochemistry and Biotechnology, Faculty of ScienceAnnamalai University
Original Contribution

DOI: 10.1007/s00394-009-0054-6

Cite this article as:
Kannappan, S. & Anuradha, C.V. Eur J Nutr (2010) 49: 101. doi:10.1007/s00394-009-0054-6

Abstract

Background

There is a growing interest in identifying putative insulin sensitizers from the herbal sources.

Aim of the study

The present study explores the effects of naringenin, a bioflavonoid, in the high fructose-induced model of insulin resistance.

Methods

Adult male Wistar rats were divided into two groups and were fed either a starch-based control diet or a high fructose diet (60 g/100 g) for 60 days. From the 16th day, rats in each group were divided into two, one of which was administered naringenin (50 mg/kg b.w.) and the other was untreated for the next 45 days. Oral glucose tolerance test (OGTT) was done on day 59. On day 60, the levels of glucose, insulin, triglycerides (TG), free fatty acids (FFA) in blood, and the activities of insulin-inducible and suppressible enzymes in the cytosolic and mitochondrial fractions of liver and skeletal muscle were assayed. The extent of protein tyrosine phosphorylation in response to insulin was determined by assaying protein tyrosine kinase (PTK) and protein tyrosine phosphatase (PTP) in liver. Liver histology with periodic acid-Schiff (PAS) staining was done to detect glycogen.

Results

Fructose administration increased the plasma levels of glucose, insulin, TG, and FFA as compared to control rats. Insulin resistance was indicated by alterations in insulin sensitivity indices. Alterations in enzyme activities and reduced glycogen content were observed in fructose-fed rats. PTP activity was higher, while PTK activity was lower suggesting reduced tyrosine phosphorylation status. Administration of naringenin improved insulin sensitivity and enhanced tyrosine phosphorylation in fructose-fed animals, while it did not affect the parameters in control diet-fed rats.

Conclusions

Naringenin improves insulin signaling and sensitivity and thereby promotes the cellular actions of insulin in this model.

Keywords

FructoseInsulin resistanceRatsNaringeninTyrosine phosphorylation

Abbreviations

HOMA

Homeostatic model assessment

FFA

Free fatty acids

ISI

Insulin sensitivity index

NAD+

Nicotinamide adenine dinucleotide

NADH

Nicotinamide adenine dinucleotide reduced

OGTT

Oral glucose tolerance test

PTP

Protein tyrosine phosphatase

PTK

Protein tyrosine kinase

PAS

Periodic acid-Schiff

QUICKI

Quantitative insulin sensitivity check index

STZ

Streptozotocin

TG

Triglycerides

Copyright information

© Springer-Verlag 2009