European Journal of Nutrition

, Volume 48, Issue 6, pp 341–347

Tachyphylaxis effects on postprandial oxidative stress and mitochondrial-related gene expression in overweight subjects after a period of energy restriction

  • Ana B. Crujeiras
  • Dolores Parra
  • Estíbaliz Goyenechea
  • Itziar Abete
  • J. Alfredo Martínez
Original Contribution

DOI: 10.1007/s00394-009-0019-9

Cite this article as:
Crujeiras, A.B., Parra, D., Goyenechea, E. et al. Eur J Nutr (2009) 48: 341. doi:10.1007/s00394-009-0019-9

Abstract

Background

Postprandially induced oxidative stress can cause damage to mitochondrial components and initiate cellular degradative processes; which are related to obesity comorbidities.

Aim of the study

This trial sought to determine whether weight loss induced by caloric restriction provides antioxidant protection to reduce the postprandial response of mitochondrial function and oxidative stress markers.

Methods

A group of overweight/obese volunteers (n = 17; 39 ±7 years, 32.5 ± 4.8 kg/m2) followed an 8-week hypocaloric diet. Volunteers provided blood samples at fasting and 2-h after a test drink (CHO: 95% E, PROT: 5% E and containing antioxidants) and these were examined for postprandial oxidative stress responses, before and after the nutritional intervention. The expression of four mitochondrial-related genes, COX15, NDUFS2, MGST2 and TNF-alfa, was measured in peripheral blood mononuclear cells (PBMC) by quantitative RT-PCR. Lipid peroxidation and nitrosative stress biomarkers, total antioxidant capacity (AOP), uric acid and glutathione peroxidase were also determined.

Results

Before nutritional treatment, the test drink induced a postprandial increase in lipid peroxidation and nitrosative stress biomarkers with a concomitant increase in the AOP. The increase in postprandial oxidative stress biomarkers was accompanied by a decrease in PBMC COX15 mRNA levels. Interestingly, after the weight loss period (−5.8 ± 2.3%), the postprandial-induced changes were lower than at the beginning of the study and involved oxidative stress biomarkers and the COX15 and MGST2 transcripts. This finding suggests the occurrence of a tachyphylactic process.

Conclusions

We demonstrate for the first time that the well-known effect of energy restriction on oxidative stress is accompanied by a tolerance mechanism on the postprandial oxidative stress response and mitochondrial function-related genes. Indeed, the COX15 and MGST2 gene expression assays in PBMC emerged as valuable nutrigenomic biomarkers of the oxidative response under energy-restriction conditions.

Keywords

Caloric restrictionCytochrome c oxidaseCOX15Oxidative stressGlutathione S-transferasePBMC gene expression

Abbreviations

AOP

Serum total antioxidant capacity

BMI

Body mass index (kg/m2)

COX15

Cytochrome c oxidase assembly protein

CR

Caloric restriction

GPx

Glutathione peroxidase

MDA

Malondialdehyde

MGST2

Microsomal glutathione S-transferase

NDUFS2

NADH-Coenzyme Q reductase

NO

Nitric oxide

NT

Nitrotyrosine

Ox-LDL

Oxidized-LDL

PBMC

Peripheral blood mononuclear cell

TNFAIP8L1

Tumor necrosis factor alfa-related gen

UA

Uric acid

UV

Ultraviolet visible

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Ana B. Crujeiras
    • 1
  • Dolores Parra
    • 1
  • Estíbaliz Goyenechea
    • 1
  • Itziar Abete
    • 1
  • J. Alfredo Martínez
    • 1
  1. 1.Department of Nutrition and Food Sciences, Physiology and ToxicologyUniversity of NavarraPamplonaSpain