European Journal of Nutrition

, Volume 45, Issue 5, pp 251–258

Defatted milled grape seed protects adriamycin-treated hepatocytes against oxidative damage

Authors

  • Victoria Valls-Belles
    • Depto. de Pediatría, Ginecología y Obstetricia Facultad de MedicinaUniversidad de Valencia
  • Mari Carmen Torres
    • Depto. de Pediatría, Ginecología y Obstetricia Facultad de MedicinaUniversidad de Valencia
    • Depto. de Biotecnología y Ciencia de los AlimentosUniversidad de Burgos
    • Área Bioquímica y Biología Molecular Facultad de CienciasUniversidad de Burgos
  • Sagrario Beltran
    • Depto. de Biotecnología y Ciencia de los AlimentosUniversidad de Burgos
  • Jesús Roman Martinez-Álvarez
    • Escuela de EnfermeríaUniversidad Complutense
  • Pilar Codoñer-Franch
    • Depto. de Pediatría, Ginecología y Obstetricia Facultad de MedicinaUniversidad de Valencia
ORIGINAL CONTRIBUTION

DOI: 10.1007/s00394-006-0591-1

Cite this article as:
Valls-Belles, V., Torres, M., Muñiz, P. et al. Eur J Nutr (2006) 45: 251. doi:10.1007/s00394-006-0591-1

Abstract

Defatted milled grape seed (DMGS) is a wine by-product obtained from the oil extraction of the grape seed that contains different types of phenolic compounds. The present study was designed to evaluate the possible protective effect of DMGS on toxicity induced by adriamycin (ADR) in isolated rat hepatocytes. The study was carried out by examining the results of lactate dehydrogenase (LDH) release to estimate cytotoxicity; the thiobarbituric acid reactant substances (TBARS) and carbonyl group levels were measured as biomarkers of oxidative stress and ATP and GSH levels as estimation of intracellular effect. The results showed that DMGS extract protects the cellular membrane from oxidative damage and consequently prevents protein and lipid oxidation. The levels of ATP and GSH changes for the ADR toxicity were restored to control value in the presence of DMGS extract. The experimental results suggest that this wine by-product may be used to decrease oxidative stress.

Keywords

DMGS extract phenolics adriamycin oxidative damage

Abbreviations

DMGS

defatted milled grape seed

LDH

lactate dehydrogenase

TBARS

thiobarbituric acid reactant substances

GSH

glutathione

ADR

adriamycin

Copyright information

© Steinkopff Verlag Darmstadt 2006