Clinical Research in Cardiology

, Volume 100, Issue 1, pp 21–27

Early outgrowth EPCs generation is reduced in patients with Buerger’s disease

Authors

  • Yoshio Katsuki
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
  • Yasuyuki Toyama
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
  • Masanori Ohtsuka
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
  • Hiroshi Koiwaya
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
  • Takaharu Nakayoshi
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
  • Tsutomu Imaizumi
    • Division of Cardio-Vascular Medicine, Department of Internal MedicineKurume University School of Medicine
Original Paper

DOI: 10.1007/s00392-010-0198-7

Cite this article as:
Katsuki, Y., Sasaki, K., Toyama, Y. et al. Clin Res Cardiol (2011) 100: 21. doi:10.1007/s00392-010-0198-7

Abstract

Background

Buerger’s disease often shows poor collateral artery generation (i.e. neovascularization) in the ischemic limbs. However, the etiology has not yet been clarified. Circulating endothelial progenitor cells (EPCs) derived from bone marrow contribute to neovascularization in the multi-step process which includes the following capacities; mobilization, differentiation, adhesion, migration, invasion and secretion.

Materials and methods

We assessed EPCs capacities in vitro and ex vivo in age- and sex-matched controls (n = 12) and patients with Buerger’s disease (n = 12), derived from peripheral blood-derived mononuclear cells (PB-MNCs).

Results

In the flow cytometry analysis, the numbers of circulating EPC (CD34+/KDR+ or CD133+/KDR+ PB-MNC) were similar between controls and patients with Buerger’s disease. Next, we cultured PB-MNC to obtain EPCs. The number of early outgrowth EPCs was significantly decreased in patients with Buerger’s disease (p < 0.005), indicating the reduced generation of early outgrowth EPCs in Buerger’s disease. However, adhesion, migration, invasion and secretion capacities were not impaired in patients with Buerger’s disease.

Conclusions

The early outgrowth EPCs generation is reduced in patients with Buerger’s disease.

Keywords

Buerger’s diseaseThromboangiitis obliteransEndothelial progenitor cellCell function

Abbreviations

EPCs

Endothelial progenitor cells

PB-MNCs

Peripheral blood-derived mononuclear cells

TAO

Thromboangiitis obliterans

ECs

Endothelial cells

KDR

Kinase domain receptor

VEGF

Vascular endothelial growth factor

FACS

Fluorescence-activated cell sorting

HUVEC

Human umbilical vein endothelial cell

Dil-acLDL

1,1′-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-labeled acetylated low-density lipoprotein

DAPI

4′,6-diamidino-2-phenylindole

b-FGF

Basic fibroblast growth factor

PDGF-BB

Platelet-derived growth factor BB

IGF

Insulin growth factor

SDF-1α

Stromal cell-derived factor-1α

ELISA

Enzyme-linked immunosorbent assay

IL-1, -6, -8

Interleukin-1, -6, -8

TGF-1α

Tumor growth factor-1α

VCAM-1

Vascular cell adhesion molecule-1

MCP-1

Monocyte chemotactic protein-1

hs-CRP

High-sensitivity C-reactive protein

m-RNA

Messenger ribonucleic acid

Copyright information

© Springer-Verlag 2010