Original Paper

Clinical Research in Cardiology

, Volume 100, Issue 1, pp 21-27

Early outgrowth EPCs generation is reduced in patients with Buerger’s disease

  • Yoshio KatsukiAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
  • , Ken-ichiro SasakiAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine Email author 
  • , Yasuyuki ToyamaAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
  • , Masanori OhtsukaAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
  • , Hiroshi KoiwayaAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
  • , Takaharu NakayoshiAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine
  • , Tsutomu ImaizumiAffiliated withDivision of Cardio-Vascular Medicine, Department of Internal Medicine, Kurume University School of Medicine

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Abstract

Background

Buerger’s disease often shows poor collateral artery generation (i.e. neovascularization) in the ischemic limbs. However, the etiology has not yet been clarified. Circulating endothelial progenitor cells (EPCs) derived from bone marrow contribute to neovascularization in the multi-step process which includes the following capacities; mobilization, differentiation, adhesion, migration, invasion and secretion.

Materials and methods

We assessed EPCs capacities in vitro and ex vivo in age- and sex-matched controls (n = 12) and patients with Buerger’s disease (n = 12), derived from peripheral blood-derived mononuclear cells (PB-MNCs).

Results

In the flow cytometry analysis, the numbers of circulating EPC (CD34+/KDR+ or CD133+/KDR+ PB-MNC) were similar between controls and patients with Buerger’s disease. Next, we cultured PB-MNC to obtain EPCs. The number of early outgrowth EPCs was significantly decreased in patients with Buerger’s disease (p < 0.005), indicating the reduced generation of early outgrowth EPCs in Buerger’s disease. However, adhesion, migration, invasion and secretion capacities were not impaired in patients with Buerger’s disease.

Conclusions

The early outgrowth EPCs generation is reduced in patients with Buerger’s disease.

Keywords

Buerger’s disease Thromboangiitis obliterans Endothelial progenitor cell Cell function