International Journal of Colorectal Disease

, Volume 27, Issue 8, pp 1039–1046

Competence and sporulation factor derived from Bacillus subtilis improves epithelial cell injury in intestinal inflammation via immunomodulation and cytoprotection

  • Kotaro Okamoto
  • Mikihiro Fujiya
  • Toshie Nata
  • Nobuhiro Ueno
  • Yuhei Inaba
  • Chisato Ishikawa
  • Takahiro Ito
  • Kentaro Moriichi
  • Hiroki Tanabe
  • Yusuke Mizukami
  • Eugene B. Chang
  • Yutaka Kohgo
Original Article

DOI: 10.1007/s00384-012-1416-8

Cite this article as:
Okamoto, K., Fujiya, M., Nata, T. et al. Int J Colorectal Dis (2012) 27: 1039. doi:10.1007/s00384-012-1416-8

Abstract

Introduction

Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. These probiotic effects are considered to be displayed through the mediation of effective molecules derived from these bacteria because live bacteria as well as their conditioned media exhibit beneficial effects in many cases. However, many of the probiotic-derived molecules which mediate such benefits have so far been poorly characterized. We previously found that competence and sporulation factor (CSF) activates the Akt and p38 MAPK pathways and protects epithelial cells from oxidant stress in the mammalian intestine. The purpose of this study is to determine the CSF effect on reducing intestinal inflammation.

Methods and results

A protein array demonstrated that CSF induced the anti-inflammatory cytokine, IL-10, and decreased the release of pro-inflammatory mediators, IL-4, IL-6 and CXCL-1, induced by TNF-α in Caco2/bbe cells. CSF also induced the cytoprotective protein Hsp 27 in Caco2/bbe cells. The histological score of intestinal inflammation in 2% dextran sodium sulfate (DSS)-treated mice with the administration of 10 nM CSF was significantly lower than that of control mice. CSF also improved the survival rate of mice treated with a lethal concentration of DSS.

Conclusion

Therefore, CSF is a potentially effective treatment for intestinal inflammation.

Keywords

Competence and sporulation factorProbioticsInflammatory bowel diseaseAnti-inflammatory cytokinePro-inflammatory cytokineIL-8IL-10CXCL-1Heat-shock proteinsChemokine

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Kotaro Okamoto
    • 1
  • Mikihiro Fujiya
    • 1
  • Toshie Nata
    • 1
  • Nobuhiro Ueno
    • 1
  • Yuhei Inaba
    • 1
  • Chisato Ishikawa
    • 1
  • Takahiro Ito
    • 1
  • Kentaro Moriichi
    • 1
  • Hiroki Tanabe
    • 1
  • Yusuke Mizukami
    • 1
  • Eugene B. Chang
    • 2
  • Yutaka Kohgo
    • 1
  1. 1.Division of Gastroenterology and Hematology/Oncology, Department of MedicineAsahikawa Medical UniversityAsahikawaJapan
  2. 2.Martin Boyer LaboratoriesUniversity of Chicago IBD Research CenterChicagoUSA