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The effect of oral tolerance on the roles of small intestinal intraepithelial lymphocytes in murine colitis induced by dextran sodium sulfate

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Backgrounds and aims

There is increasing evidence that gut-derived intraepithelial lymphocytes have potent cytolytic and immunoregulatory functions, which they use to sustain epithelial integrity. The aims of this study were to investigate the roles of small intestinal intraepithelial lymphocytes (SI-IELs) in oral tolerance and dextran sodium sulfate (DSS)-induced colitis.

Methods

SI-IELs or sorted γδ T cells from untreated, colitis, and colitis-extracted protein (CEP)-fed colitis mice were adoptively transferred to BALB/c mice; colitis was then induced with DSS. Cytokines were analyzed in sera from mice and culture supernatants.

Results

Transfer of SI-IELs or sorted γδ T cells from untreated and colitis mice all alleviated experimental colitis. Mice orally administered with five low doses of CEP showed less severe symptoms and histological injury. SI-IELs from CEP-fed colitis mice more significantly ameliorated colitis than those from control mice (weight, 94.1 ± 2.5% vs. 89.8 ± 2.6%, p < 0.05; disease activity index, 7.2 ± 1.2 vs. 8.7 ± 1.9, p < 0.05; histological scores, 22.1 ± 2.8 vs. 25.7 ± 2.1, p < 0.05, n = 8 per group); however, not did SI-γδ IELs from CEP-fed colitis mice. Alleviation of colitis was accompanied by an increase of TGF-β1 secretion and no change of IFN-γ in sera and culture supernatants. The level of serum TGF-β1 was negatively related to the severity of colitis.

Conclusions

The protective effects of SI-IELs in DSS-induced colitis were partly accomplished by γδ T cells and could be mediated by TGF-β but were not associated with IFN-γ. Oral tolerance strengthens the suppressive effects of regulatory subsets in SI-IELs.

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Authors and Affiliations

  1. Department of Gastroenterology, the Affiliated Hospital, Hangzhou Normal University College of Medicine, Hangzhou, People’s Republic of China

    Yuefang Ye

  2. Department of Gastroenterology, the First Affiliated Hospital, Zhejiang University College of Medicine, No. 79 Qingchun Road, Hangzhou, Zhejiang, 310003, People’s Republic of China

    Min Yue, Xi Jin, Shaohua Chen & Youming Li

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  1. Yuefang Ye
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  2. Min Yue
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  3. Xi Jin
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  4. Shaohua Chen
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  5. Youming Li
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Corresponding author

Correspondence to Youming Li.

Additional information

This work was supported by Grant Y2110864 from Natural Science Foundation of Zhejiang Province.

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Ye, Y., Yue, M., Jin, X. et al. The effect of oral tolerance on the roles of small intestinal intraepithelial lymphocytes in murine colitis induced by dextran sodium sulfate. Int J Colorectal Dis 27, 583–593 (2012). https://doi.org/10.1007/s00384-011-1354-x

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