International Journal of Colorectal Disease

, Volume 25, Issue 1, pp 63–69

Colorectal cancer in Iran: immunohistochemical profiles of four mismatch repair proteins

  • Mahsa Molaei
  • Babak Khoshkrood Mansoori
  • Somayeh Ghiasi
  • Fatemeh Khatami
  • Hamid Attarian
  • MohammadReza Zali
Original Article

DOI: 10.1007/s00384-009-0784-1

Cite this article as:
Molaei, M., Mansoori, B.K., Ghiasi, S. et al. Int J Colorectal Dis (2010) 25: 63. doi:10.1007/s00384-009-0784-1

Abstract

Purpose

The aim of the present study was to determine the profile of mismatch repair (MMR) defects in Iranian colorectal cancer patients by using immunohistochemical staining for products of four MMR genes: MLH1, MSH2, PMS2, and MSH6.

Methods

Tissue samples of 343 patients were immunostained for MLH1, MSH2, PMS2, and MSH6. Clinical and family history and survival data were compared between normal and abnormal staining patterns.

Results

Fourteen percent of the patients had abnormal nuclear staining for MMR proteins. MLH1 was absent in four, MLH1/PMS2 in 15, PMS2 in five, MSH2 in 12, and MSH2/MSH6 in 12 patients. These tumors were more proximal, had a nonsignificant better survival, and were more associated with positive family history. Estimation of this study of prevalence of hereditary nonpolyposis colorectal cancer in Iran was 5.5% of the total colorectal cancers.

Conclusions

Along with the recommendations of the National Institute of Cancer, we recommend immunohistochemistry staining for MLH1, MSH2, PMS2, and MSH6 for determining the eligibility of patients for mutation analysis of MMR genes.

Keywords

DNA mismatch repair Hereditary nonpolyposis colorectal neoplasm MLH1 protein MSH2 protein MSH6 protein PMS2 protein 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Mahsa Molaei
    • 1
  • Babak Khoshkrood Mansoori
    • 2
  • Somayeh Ghiasi
    • 2
  • Fatemeh Khatami
    • 2
  • Hamid Attarian
    • 3
  • MohammadReza Zali
    • 2
  1. 1.Department of Pathology, Research Institute for Gastroenterology and Liver Diseases, Taleghani HospitalShahid Beheshti University M.C.TehranIran
  2. 2.Research Institute for Gastroenterology and Liver Diseases, Taleghani HospitalShahid Beheshti University M.C.TehranIran
  3. 3.Department of Medical Oncology and Hematology, Taleghani HospitalShahid Beheshti University M.C.TehranIran

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