Identification of IMPDH2 as a tumor-associated antigen in colorectal cancer using immunoproteomics analysis

  • Yujun He
  • Zhirong Mou
  • Wanlin Li
  • Baohua Liu
  • Tao Fu
  • Shong Zhao
  • Debing Xiang
  • Yuzhang Wu
Original Article

DOI: 10.1007/s00384-009-0759-2

Cite this article as:
He, Y., Mou, Z., Li, W. et al. Int J Colorectal Dis (2009) 24: 1271. doi:10.1007/s00384-009-0759-2

Abstract

Background and aims

Sera from cancer patients contain tumor-specific autoantibodies directly against antigenic proteins. The identification of tumor autoantigens may have utility in cancer diagnosis, prognosis, and therapy. In this study, we used immunoproteomics analysis to identify tumor proteins that elicit humoral response in colorectal cancer (CRC).

Materials and methods

The CRC cell line HCT116 was used as a source of proteins for two-dimensional polyacrylamide gel electrophoresis and subsequent Western blot analysis in which individual serum from patients with CRC was analyzed for autoantibodies. Proteins that specifically react with sera from cancer patients were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometric analysis. In addition, the selected protein expression in tumor tissues collected from 40 patients with CRC were assessed by immunohistochemistry.

Results

An autoantibody against inosine monophosphate dehydrogenase II (IMPDH2) identified by mass spectrometry was detected in eight out of 25 patients with CRC. However, none of the 15 healthy controls demonstrated autoantibody to IMPDH2.The expression of IMPDH2 in tumor tissue was significantly higher in patients with CRC than that in healthy subjects.

Conclusions

The result confirmed that the immunoproteomics analysis holds considerable promise for the discovery of tumor-associated antigens. IMPDH2 may be a protein biomarker and novel therapeutic target in CRC.

Keywords

Colorectal cancer Immunoproteomics analysis Tumor-associated antigens Inosine monophosphate dehydrogenase II (IMPDH2) 

Abbreviations

CRC

colorectal cancer

2-D PAGE

two-dimensional polyacrylamide gel electrophoresis

PVDF

polyvinylidene fluoride

MALDI-TOF

matrix-assisted laser desorption ionization time-of-flight

TAAs

tumor-associated antigens

IMPDH2

inosine monophosphate dehydrogenase II

PTMs

post-translational modifications

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Yujun He
    • 1
    • 2
  • Zhirong Mou
    • 2
  • Wanlin Li
    • 2
  • Baohua Liu
    • 1
  • Tao Fu
    • 1
  • Shong Zhao
    • 1
  • Debing Xiang
    • 3
  • Yuzhang Wu
    • 2
  1. 1.Department of General Surgery, Daping Hospital and Research Institute of SurgeryThird Military Medical UniversityChongqingChina
  2. 2.Institute of Immunology of PLAThird Military Medical UniversityChongqingChina
  3. 3.Cancer Center, Daping Hospital and Research Institute of SurgeryThird Military Medical UniversityChongqingChina

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