International Journal of Colorectal Disease

, Volume 23, Issue 10, pp 947–955

Inflammation modulates fibronectin isoform expression in colonic lamina propria fibroblasts (CLPF)

  • Julia Brenmoehl
  • Werner Falk
  • Michael Göke
  • Jürgen Schölmerich
  • Gerhard Rogler
Original Article

DOI: 10.1007/s00384-008-0523-z

Cite this article as:
Brenmoehl, J., Falk, W., Göke, M. et al. Int J Colorectal Dis (2008) 23: 947. doi:10.1007/s00384-008-0523-z

Abstract

Background

Migration of colonic lamina propria fibroblasts (CLPF) plays an important role during mucosal wound healing as well as fibrosis and fistula formation in Crohn’s disease (CD). Recently, we showed that the migratory potential of CD-CLPF was significantly reduced compared to control CLPF. Fistula-derived CD-CLPF migrated less and fibrosis-CLPF more than CLPF from inflamed CD mucosa. These changes in migratory behavior were associated with changes in production of the migration-inducing fibronectin (FN) isoforms ED-A and ED-B. A permanent reduction of the migratory potential of CLPF was mediated by IFN-γ and tumor necrosis factor (TNF) modulate FN isofom expression in CLPF and thereby might regulate CLPF migration.

Materials and methods

Control CLPF were incubated for 72 h with IFN-γ, TNF, IFN-γ plus TNF, or TGF-β1. Messenger RNA (mRNA) was isolated and expression of FN and isoforms ED-A and ED-B was quantified by real-time polymerase chain reaction. FN, ED-A, and ED-B were investigated by Western blotting. FN receptor integrin α5β1 was analyzed by FACS.

Results

No difference was found for the surface display of integrin α5β1 between stimulated and non-stimulated cells. In TGF-β1 incubated CLPF mRNA amount of FN and isoforms ED-A and ED-B was slightly increased. IFN-γ only decreased FN in CLPF, TNF significantly reduced FN-mRNA by 40%, FN ED-A mRNA by 25%, and ED-B mRNA by 50%. The TNF-mediated mRNA downregulation resulted in a decreased protein amount as revealed by Western blotting.

Conclusion

Cytokines such as IFN-γ, TNF, and TGF-β1 modulate the production of fibronectin isoforms. Our data indicate that inflammation-induced modulation of FN-isoform production is involved in the alterations of migratory potential of CLPF isolated from CD mucosa.

Keywords

Fibronectin isoformsIntegrin α5β1Colonic lamina propria fibroblastsCytokines

Abbreviations

AIDA

Advanced Image Data Analyser

CLPF

colonic lamina propria fibroblasts

CD

Cohn’s disease

DMEM

Dulbecco’s Modified Eagle’s medium

ED-A

extra domain A

ED-B

extra domain B

IIICS

type III connecting segment

EDTA

ethylendiaminetetraacetic acid

FCS

fetal calf serum

FN

fibronectin

GAPDH

glycerinaldehyde-3-phosphate dehydrogenase

IBD

inflammatory bowel disease

IFN-γ

interferon-γ

PBS

phosphate-buffered saline

SDS

sodium dodecyl sulfate

TNF

tumor necrosis factor

TGF-β1

transforming growth factor-β1

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Julia Brenmoehl
    • 2
  • Werner Falk
    • 1
  • Michael Göke
    • 4
  • Jürgen Schölmerich
    • 1
  • Gerhard Rogler
    • 3
  1. 1.Department of Internal Medicine IUniversity of RegensburgRegensburgGermany
  2. 2.Department of Internal Medicine IIUniversity of JenaJenaGermany
  3. 3.Department of Internal MedicineUniversity of ZurichZurichSwitzerland
  4. 4.Department of Internal MedicineMalteser Hospital Bonn/Rhein-SiegBonnGermany