International Journal of Colorectal Disease

, Volume 22, Issue 7, pp 777–782

Assessment of microsatellite instability in colorectal carcinoma at an Indian center

  • Vijay Pandey
  • Jyothi S. Prabhu
  • Payal K.
  • Veena Rajan
  • C. Deepak
  • Shubhada Barde
  • P. Jagannath
  • Anita Borges
  • Sridhar T. S.
Original Article

DOI: 10.1007/s00384-006-0241-3

Cite this article as:
Pandey, V., Prabhu, J.S., K., P. et al. Int J Colorectal Dis (2007) 22: 777. doi:10.1007/s00384-006-0241-3

Abstract

Aim

The purpose of the present study was to evaluate the frequency of microsatellite instability (MSI) in colorectal cancers in an Indian cohort.

Materials and methods

Paraffin embedded tissue samples of colorectal cancers from 46 patients were assessed for mismatch repair protein expression (hMLH1 and hMSH2) by immunohistochemistry. Subsequently, MSI analysis was done after PCR amplification of five Bethesda markers.

Results

Amongst 46 cases studied, only 5 patients (10.8%) showed MSI. Out of these, two (4.3%) had high microsatellite instability (MSI-H) and three (6.5%) showed low microsatellite instability (MSI-L). Out of 46 cases, 41 were microsatellite stable (MSS). In the 46 cases tested by immunohistochemistry, 7 (15.7%) showed the absence of hMLH1 and 1 case showed the absence of hMSH2.

Conclusion

Our study indicates a similar rate of incidence of MSI in colorectal cancers in the Indian cohort compared to the West (10–15%) despite lower incidence of colorectal cancers and predominance of rectosigmoid tumors in the Indian population.

Keywords

Microsatellite instabilityColorectal cancerhMLH1hMSH2ImmunohistochemistryPCR

Abbreviations

MSI

microsatellite instability

CRC

colorectal cancer

gDNA

genomic DNA

MSS

microsatellite stable

MSI-L

microsatellite instability low

MSI-H

microsatellite instability high

HNPCC

hereditary non-polyposis colorectal cancer

H & E

hematoxylin and eosin

IHC

immunohistochemistry

CAP

College of American Pathologists

hMLH1

human mutL homolog 1

hMSH2

human mutS homolog 2

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Vijay Pandey
    • 1
  • Jyothi S. Prabhu
    • 1
  • Payal K.
    • 1
  • Veena Rajan
    • 1
  • C. Deepak
    • 2
  • Shubhada Barde
    • 2
  • P. Jagannath
    • 2
  • Anita Borges
    • 2
  • Sridhar T. S.
    • 1
  1. 1.Triesta Sciences, IPHCR BuildingSt. John’s Medical College CampusBangaloreIndia
  2. 2.Asian Institute of OncologyS.L. Raheja HospitalMumbaiIndia