International Journal of Colorectal Disease

, Volume 21, Issue 2, pp 114–120

Decreased levels of interleukin-12p40 in the serum of patients with Whipple’s disease

  • A. Kalt
  • T. Schneider
  • S. Ring
  • J. Hoffmann
  • M. Zeitz
  • A. Stallmach
  • D. H. Persing
  • T. Marth
Original Article

DOI: 10.1007/s00384-005-0778-6

Cite this article as:
Kalt, A., Schneider, T., Ring, S. et al. Int J Colorectal Dis (2006) 21: 114. doi:10.1007/s00384-005-0778-6

Abstract

Background

An impaired production of interleukin (IL)-12 and T cell interferon-γ (IFN-γ) of in vitro stimulated monocytes has been discussed as a pathogenic factor in Whipple’s disease (WD). It is unclear whether this defect of cellular immunity is translated to the humoral immune system and to serum correlates.

Methods

We analyzed the serum of 40 patients with Whipple’s disease in various degrees of disease activity by sandwich enzyme-linked immunosorbent assay for differences in cytokine and cell adhesion molecule concentrations compared with age- and sex-matched controls.

Results

We observed a highly significant reduction of IL-12p40 levels (patients, 0.18±0.05 ng/ml (mean±SEM); controls, 3.19±0.39 ng/ml; p<0.01) in all stages of disease activity, whereas the concentration of IL-12p70 was comparable with controls. Furthermore, we observed a slight decrease in tumour necrosis factor α (TNF-α) concentrations in the serum of patients (patients, 6.36±0.90 pg/ml; controls, 10.5±1.23 pg/ml; p<0,05). The levels of other cytokines such as IFN-γ, IL-2, IL-13 and transforming growth factor β, as well as soluble cell adhesion molecules lymphocyte function-associated antigen 3 and intercellular adhesion molecule 1, were not significantly different compared with controls. Levels of immunoglobulin G2 (IgG2) measured in the serum of WD patients were below normal in 24 of 29 patients and were even below the 95% confidence interval in 10 patients.

Conclusion

Our data demonstrate a persistent defect of the cellular immune response with decreased serum concentrations of IL-12p40 and TNF-α and decreased IgG2 levels in a large group of WD patients. These data support as in vivo finding the results obtained in previous investigations with stimulated monocytes/lymphocytes. The isolated decrease in IL-12p40 may hint at possible defects in the IL-12/IFN-γ promoter system.

Keywords

Whipple’s diseaseImmunologyCytokinesImmunoglobulin

Abbreviations

ANOVA

analysis of variance

ELISA

enzyme-linked immunosorbent assay

ICAM

intercellular adhesion molecule

ICSBP

interferon consensus sequence binding protein

IFN-γ

interferon gamma

IgG2

immunoglobulin G2

IL

interleukin

LFA

lymphocyte function-associated antigen

LPS

lipopolysaccharide

SD

standard deviation

TGF-β

transforming growth factor beta

TNF-α

tumor necrosis factor alpha

WD

Whipple’s disease

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • A. Kalt
    • 1
  • T. Schneider
    • 2
  • S. Ring
    • 3
  • J. Hoffmann
    • 2
  • M. Zeitz
    • 2
  • A. Stallmach
    • 4
  • D. H. Persing
    • 5
  • T. Marth
    • 6
  1. 1.Department of DermatologyThe University of the SaarlandHomburg/SaarGermany
  2. 2.Department of Internal Medicine ICharité, Campus Benjamin Franklin HospitalBerlinGermany
  3. 3.Department of GastroenterologyDeutsche Klinik für DiagnostikWiesbadenGermany
  4. 4.Department of GastroenterologyMarienhospital, Katholische Kliniken Essen-NordwestEssenGermany
  5. 5.Department of Clinical MicrobiologyMayo ClinicRochesterUSA
  6. 6.Department of Internal MedicineSt. Josef HospitalZell/MoselGermany