International Journal of Colorectal Disease

, Volume 20, Issue 2, pp 161–164

Differential expression of anti-apoptotic protein Bcl-2 in keratinizing versus non-keratinizing squamous cell carcinoma of the anus

Authors

    • Division of Radiation OncologyUniversity Hospital Geneva
  • Marie-Anne Bründler
    • Division of Clinical PathologyUniversity Hospital Geneva
  • Pascal Gervaz
    • Department of SurgeryUniversity Hospital Geneva
Original Article

DOI: 10.1007/s00384-004-0651-z

Cite this article as:
Allal, A.S., Bründler, M. & Gervaz, P. Int J Colorectal Dis (2005) 20: 161. doi:10.1007/s00384-004-0651-z

Abstract

Background

Histologically, tumors of the anal region are either keratinizing (K) or non-keratinizing (NK) squamous cell carcinomas (SCCA). We hypothesized that these two variants might represent, not only morphologically, but also biologically, dissimilar malignancies. The present study was undertaken to compare the expression of apoptosis-regulating proteins Bcl-2 and p53 in K versus NK SCCA.

Methods

We performed an immunohistochemical analysis on 98 pre-treatment biopsies of patients with anal canal cancers. Tissue sections were examined immunohistochemically for expression of proteins Bcl-2 (clone 124, DAKO, 1:100) and p53 (clone DO7, DAKO, 1:200). Expression of p53 and Bcl-2 was considered positive when >5% of tumor cells were stained. Tumor histology was correlated with protein expression as well as with other clinical variables.

Results

There were 64 NK and 34 K SCC. The proportion of Bcl-2 positive tumors was statistically higher in NK carcinomas (51.5 vs. 23.5%, p=0.009). In addition, women were more likely than men to present with NK carcinomas (71 vs. 45%, p=0.03) as well as with Bcl-2 positive tumors (47 vs. 29%, p=0.05). The more distal the tumor is (anal margin), the more frequently the keratinizing subtype is observed (87 vs. 23%, p=0.0002). By contrast, there was no correlation between p53 and tumor histology (p=0.83).

Conclusions

Our data demonstrate that non-keratinizing and keratinizing SCCA differ in their Bcl-2 expression. In addition, significant differences were observed in the distribution of these two histological subtypes according to gender and tumor sublocation. These findings may indicate possible differences in the carcinogenesis process of these two histological subtypes.

Keywords

Anal cancerImmunohistochemistryKeratinizingP53Bcl-2

Copyright information

© Springer-Verlag 2004