Original Article

Pediatric Surgery International

, Volume 26, Issue 1, pp 79-84

First online:

Amniotic fluid stem cell migration after intraperitoneal injection in pup rats: implication for therapy

  • Marco GhionzoliAffiliated withDepartment of Surgery, Institute of Child Health
  • , Mara CananziAffiliated withDepartment of Surgery, Institute of Child Health
  • , Augusto ZaniAffiliated withDepartment of Surgery, Institute of Child Health
  • , Carlo Alberto RossiAffiliated withDepartment of Surgery, Institute of Child Health
  • , Francesco Fascetti LeonAffiliated withDepartment of Surgery, Institute of Child Health
  • , Agostino PierroAffiliated withDepartment of Surgery, Institute of Child Health
  • , Simon EatonAffiliated withDepartment of Surgery, Institute of Child Health
  • , Paolo De CoppiAffiliated withDepartment of Surgery, Institute of Child Health Email author 

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Abstract

Purpose

Despite being commonly used in clinical practice, the intraperitoneal (i.p.) route has been rarely used for cell delivery. We evaluated the capacity of amniotic fluid stem (AFS) cells, administered i.p., to diffuse systemically and to integrate into tissues of healthy newborn rats.

Methods

AFS cells were obtained from pregnant GFP + Sprague–Dawley rats by c-kit selection. Wild-type Sprague–Dawley newborn rats were divided into two groups receiving i.p.: (1) 2 × 106 AFS cells (n = 12); (2) of phosphate buffer saline (PBS) (n = 2) at 24 and 48 h after birth. Animals were either killed at 96 h of life, and organs collected for gfp amplification, or at 3 weeks of life and tissues isolated for green fluorescence protein (GFP) immunofluorescence.

Results

No adverse effects were observed after i.p. injection of PBS or AFS cells. Gfp was amplified in at least one organ in all rats injected with AFS cells except one (11/12). The intestine was the organ found most frequently positive (67%) followed by liver (25%), spleen (16%), heart (16%), lungs (16%), femur (8%) and brain (0%). Immunohistochemistry confirmed PCR results.

Conclusion

In the short term, the i.p. administration of AFS cells, is a safe procedure and allows their migration, homing and integration into various organs of healthy newborn rats.

Keywords

Pup rat Amniotic fluid stem cell Intraperitoneal injection Cell therapy