Child's Nervous System

, 27:1877

MGMT promoter gene methylation in pediatric glioblastoma: analysis using MS-MLPA

  • Ji Yeoun Lee
  • Chul-Kee Park
  • Sung-Hye Park
  • Kyu-Chang Wang
  • Byung-Kyu Cho
  • Seung-Ki Kim
Original Paper

DOI: 10.1007/s00381-011-1525-7

Cite this article as:
Lee, J.Y., Park, CK., Park, SH. et al. Childs Nerv Syst (2011) 27: 1877. doi:10.1007/s00381-011-1525-7

Abstract

Purpose

Promoter methylation of the O6-methylguanine-DNA-methyltransferase (MGMT) gene is widely recognized as an important predictive factor in the treatment of glioblastoma (GBM) patients with temozolomide. However, data regarding the methylation status of the MGMT promoter in pediatric GBM are yet to be elucidated.

Methods

Nineteen tissue samples of pediatric GBM were evaluated for the MGMT promoter methylation status using methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA). Methylation status was also evaluated using methylation-specific polymerase chain reaction (MSP) for 17 of the 19 patients. The correlation between MGMT promoter methylation and clinical outcome was assessed.

Results

Three of the 19 patients (16%) showed methylation of the MGMT promoter, according to MS-MLPA, as did 1 of the 17 (6%), according to MSP. The methylation status did not seem to have a definite effect on clinical outcome.

Conclusions

Pediatric GBMs rarely have methylated MGMT promoters. With a better clinical outcome and lower methylation rate than their adult counterparts, it may be suggested that, for pediatric GBM, MGMT promoter methylation does not play a significant role as a prognostic factor.

Keywords

Glioblastoma Pediatric MGMT Methylation MS-MLPA 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ji Yeoun Lee
    • 1
  • Chul-Kee Park
    • 1
  • Sung-Hye Park
    • 2
  • Kyu-Chang Wang
    • 1
  • Byung-Kyu Cho
    • 1
  • Seung-Ki Kim
    • 1
  1. 1.Division of Pediatric Neurosurgery, Department of NeurosurgerySeoul National University Children’s Hospital, Seoul National University College of MedicineSeoulRepublic of Korea
  2. 2.Department of PathologySeoul National University Children’s Hospital, Seoul National University College of MedicineSeoulRepublic of Korea