Child's Nervous System

, Volume 26, Issue 11, pp 1613–1618

MGMT gene promoter methylation in pediatric glioblastomas

  • Arti Srivastava
  • Ayushi Jain
  • Prerana Jha
  • Vaishali Suri
  • Mehar Chand Sharma
  • Supriya Mallick
  • Tarun Puri
  • Deepak Kumar Gupta
  • Aditya Gupta
  • Chitra Sarkar
Original Paper

DOI: 10.1007/s00381-010-1214-y

Cite this article as:
Srivastava, A., Jain, A., Jha, P. et al. Childs Nerv Syst (2010) 26: 1613. doi:10.1007/s00381-010-1214-y

Abstract

Purpose

Relatively few studies have been performed on molecular properties of pediatric glioblastoma multiforme (GBM). Methylation of DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT) promoter region has been associated with favorable prognosis and prolonged survival in adult GBM patients treated with temozolomide (TMZ). We explored the frequency of MGMT gene promoter methylation in pediatric glioblastomas and compared it with the known molecular alterations in p53.

Methods

Twenty pediatric GBM cases were selected. MGMT promoter methylation was assessed by methylation specific PCR. p53 expression was determined by immunohistochemistry.

Results

MGMT gene promoter methylation was observed in 50% of pediatric glioblastomas. p53 protein expression was detected in 60% of cases. Seventy percent of cases with methylated MGMT promoter were p53 immunopositive.

Conclusions

The frequency of MGMT gene promoter methylation in pediatric GBMs was similar to adult GBM patients. The pediatric GBMs should also be investigated for MGMT promoter methylation to identify a subset of patients likely to benefit from TMZ therapy. p53 protein overexpression was more common in pediatric primary GBMs. To the best of our knowledge this is only the second study on MGMT gene promoter methylation status in pediatric GBMs.

Keywords

Glioblastoma multiforme Pediatric tumor MGMT methylation p53 

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Arti Srivastava
    • 1
  • Ayushi Jain
    • 1
  • Prerana Jha
    • 1
  • Vaishali Suri
    • 1
  • Mehar Chand Sharma
    • 1
  • Supriya Mallick
    • 2
  • Tarun Puri
    • 2
  • Deepak Kumar Gupta
    • 3
  • Aditya Gupta
    • 3
  • Chitra Sarkar
    • 1
  1. 1.Department of PathologyAll India Institute of Medical Sciences (AIIMS)New DelhiIndia
  2. 2.Department of RadiotherapyAll India Institute of Medical Sciences (AIIMS)New DelhiIndia
  3. 3.Department of NeurosurgeryAll India Institute of Medical Sciences (AIIMS)New DelhiIndia