Child's Nervous System

, Volume 26, Issue 3, pp 279–283

Upregulation of mir-221 and mir-222 in atypical teratoid/rhabdoid tumors: potential therapeutic targets

  • Simone Treiger Sredni
  • Maria de Fátima Bonaldo
  • Fabrício Falconi Costa
  • Chiang-Ching Huang
  • Christopher Allan Hamm
  • Veena Rajaram
  • Tadanori Tomita
  • Stewart Goldman
  • Jared Marshall Bischof
  • Marcelo Bento Soares
Brief Communication

DOI: 10.1007/s00381-009-1028-y

Cite this article as:
Sredni, S.T., Bonaldo, M..F., Costa, F.F. et al. Childs Nerv Syst (2010) 26: 279. doi:10.1007/s00381-009-1028-y

Abstract

Purpose

The aim of this study is to search for new therapeutic targets for atypical teratoid–rhabdoid tumors (ATRT).

Methods

To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain.

Results

MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27Kip1. Here, we demonstrated the negative regulation of p27Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry.

Conclusion

As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.

Keywords

Atypical teratoid/rhabdoid tumors miR221 miR222 p27Kip1 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Simone Treiger Sredni
    • 1
  • Maria de Fátima Bonaldo
    • 1
  • Fabrício Falconi Costa
    • 1
  • Chiang-Ching Huang
    • 2
  • Christopher Allan Hamm
    • 1
    • 3
  • Veena Rajaram
    • 4
  • Tadanori Tomita
    • 5
  • Stewart Goldman
    • 6
  • Jared Marshall Bischof
    • 1
  • Marcelo Bento Soares
    • 1
  1. 1.Cancer Biology and Epigenomics Program, Children’s Memorial Research Center, Department of Pediatrics, Feinberg School of Medicine, Children’s Memorial Hospital ChicagoUSA
  2. 2.Department of Preventive Medicine, Feinberg School of MedicineNorthwestern UniversityChicagoUSA
  3. 3.Interdisciplinary Graduate Program in GeneticsUniversity of IowaIowa CityUSA
  4. 4.Division of Anatomic PathologyChildren’s Memorial HospitalChicagoUSA
  5. 5.Division of Pediatric NeurosurgeryChildren’s Memorial HospitalChicagoUSA
  6. 6.Division of Hematology/Oncology/TransplantationChildren’s Memorial HospitalChicagoUSA