Child's Nervous System

, Volume 25, Issue 1, pp 13–20

Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis

Authors

  • Jian-Jun Zhao
    • Peking Union Medical CollegeTsinghua University
    • Department of Genetics, National Research Institute for Family Planning, Peking Union Medical CollegeTsinghua University
    • H. Lee Moffitt Cancer Center and Research InstituteUniversity of South Florida
  • Juhua Yang
    • Department of Genetics, National Research Institute for Family Planning, Peking Union Medical CollegeTsinghua University
    • Biomedical Engineering CenterFujian Medical University
  • Jianhong Lin
    • Peking Union Medical CollegeTsinghua University
    • H. Lee Moffitt Cancer Center and Research InstituteUniversity of South Florida
  • Nan Yao
    • Peking Union Medical CollegeTsinghua University
    • Department of Genetics, National Research Institute for Family Planning, Peking Union Medical CollegeTsinghua University
  • Yihua Zhu
    • Department of OphthalmologyThe First Affiliated Hospital of Fujian Medical University
  • Jianlong Zheng
    • Department of OphthalmologyThe First Affiliated Hospital of Fujian Medical University
  • Jianhua Xu
    • Biomedical Engineering CenterFujian Medical University
  • Jin Q. Cheng
    • H. Lee Moffitt Cancer Center and Research InstituteUniversity of South Florida
    • Key Laboratory of Infection and Oncology, Molecular Medicine Research Center of Fujian Medical UniversityFujian Province University
    • Peking Union Medical CollegeTsinghua University
    • Department of Genetics, National Research Institute for Family Planning, Peking Union Medical CollegeTsinghua University
Original Paper

DOI: 10.1007/s00381-008-0701-x

Cite this article as:
Zhao, J., Yang, J., Lin, J. et al. Childs Nerv Syst (2009) 25: 13. doi:10.1007/s00381-008-0701-x

Abstract

Introduction

MicroRNAs (miRNAs) are small (∽22 nucleotides) regulatory RNAs which play fundamental roles in many human diseases, including cancer. There is no report on the miRNA expression profile of retinoblastoma.

Methods

This work was undertaken to identify differentially expressed miRNAs in human retinoblastoma tissues by microRNA microarray technique, and some miRNAs were verified using northern blot analysis and the in situ hybridization method.

Results

A cluster of microRNAs was identified as highly expressed in retinoblastoma, including hsa-miR-494, hsa-let-7e, hsa-miR-513–1, hsa-miR-513–2, hsa-miR-518c*, hsa-miR-129–1, hsa-miR-129–2, hsa-miR-198, hsa-miR-492, hsa-miR-498, hsa-miR-320, hsa-miR-503, and hsa-miR-373*.

Conclusion

These miRNAs are the first to be reported for human retinoblastoma and may play significant roles in regulating tumor genesis.

Keywords

MicroRNAsRetinoblastomaMicroarrayIn situ hybridizationLocked nucleic acid

Copyright information

© Springer-Verlag 2008