Child's Nervous System

, 22:475

Monitoring the growth effect of xenotransplanted human medulloblastoma in an immunocompromised mouse model using in vitro and ex vivo green fluorescent protein imaging

Authors

  • Shih-Hwa Chiou
    • Department of Education and ResearchThe Neurological Institute
    • Institute of Clinical MedicineNational Yang-Ming University
  • Chung-Lan Kao
    • Department of Physical Medicine & RehabilitationThe Neurological Institute
  • Han-Tso Lin
    • Department of Education and ResearchThe Neurological Institute
    • Institute of Anatomy and Cell BiologyNational Yang-Ming University
  • Wen-Ser Tseng
    • Division of Pediatric Neurosurgery, The Neurological InstituteTaipei Veterans General Hospital and National Yang-Ming University
  • Ren-Shyan Liu
    • Department of Nuclear MedicineTaipei Veterans General Hospital and National Yang-Ming University
  • Chen-Fun Chung
    • Institute of Anatomy and Cell BiologyNational Yang-Ming University
  • Hung-Hai Ku
    • Institute of Anatomy and Cell BiologyNational Yang-Ming University
  • Ching-Po Lin
    • Institute of NeuroscienceNational Yang-Ming University
    • Division of Pediatric Neurosurgery, The Neurological InstituteTaipei Veterans General Hospital and National Yang-Ming University
Original Paper

DOI: 10.1007/s00381-005-0026-y

Cite this article as:
Chiou, S., Kao, C., Lin, H. et al. Childs Nerv Syst (2006) 22: 475. doi:10.1007/s00381-005-0026-y

Abstract

Introduction

Medulloblastoma (MB) is one of the most common malignant brain tumors in children. It is a radiosensitive tumor. At 5 years after radical surgical excision and craniospinal axis irradiation, the tumor-free survival rate is from 50 to 70% [Halperin EC, Constine LS, Tarbell NJ, Kun LE. Pediatric radiation oncology (2005)].

Case report

In this study, we established xenotransplanted human MB (hMB) cells — isochromosome 17q — in a severe combined immunodeficiency (SCID) mouse model. We further transduced green fluorescent protein (GFP) into hMB cells to evaluate these hMB cells grafted in SCID mice.

Results

The result of an ex vivo GFP imaging system showed that a small lesion of the third-week-hMB-transplanted graft presented “green” signals with a clear tumor margin before any tumor-related symptoms were noted. We also demonstrated that the tumor progression could be monitored by GFP imaging for up to 12 weeks post-transplantation.

Conclusions

This novel approach of GFP imaging assessment provides more accurate information of tumor status for experimental brain tumor studies. Because MB is sensitive to radiation and also response to chemotherapy, this SCID mouse model will be helpful for preclinical studies in the future.

Keywords

Medulloblastoma Green fluorescent protein imaging SCID mouse model

Copyright information

© Springer-Verlag 2006