Heart and Vessels

, Volume 22, Issue 4, pp 254–260

Aldosterone induces circadian gene expression of clock genes in H9c2 cardiomyoblasts

  • Kyoe Tanaka
  • Naoto Ashizawa
  • Hiroaki Kawano
  • Osami Sato
  • Shinji Seto
  • Eijun Nishihara
  • Hideyuki Terazono
  • Shojiro Isomoto
  • Kazuyuki Shinohara
  • Katsusuke Yano
ORIGINAL ARTICLE

DOI: 10.1007/s00380-006-0968-3

Cite this article as:
Tanaka, K., Ashizawa, N., Kawano, H. et al. Heart Vessels (2007) 22: 254. doi:10.1007/s00380-006-0968-3

Abstract

We examined mRNA expression of the clock genes (Per1, Per2, and Bmal1) and PAI-1 (plasminogen activator inhibitor-1) after aldosterone treatment every 4 h up to 48 h in H9c2 cardiomyoblasts by reverse transcription–polymerase chain reaction. To block the MR (mineralocorticoid receptor), the MR antagonist, spironolactone, was added to the medium 1 h before aldosterone treatment. Aldosterone induced an initial increase and rhythmic expression of Per1, while spironolactone attenuated the acute increase in Per1 mRNA induced by aldosterone. On the other hand, aldosterone did not increase the Per2 mRNA in the acute phase, but thereafter induced a rhythmic expression of Per2. Aldosterone also induced rhythmic expression of Bmal1, a positive element of the clock genes. The rhythm of Bmal1 mRNA was anti-phase of that of Per2 mRNA. Aldosterone induced an acute increase in PAI-1 mRNA, but did not induce rhythmic expression of PAI-1. The present study demonstrated first that aldosterone regulates expression of the clock genes Per1, Per2, and Bmal1, and increases PAI-1 expression in H9c2 cardiomyoblasts. Second, an acute increase in Per1 mRNA after aldosterone treatment is mediated through MR. Third, clock genes are not related to PAI-1 expression in H9c2 cardiomyoblasts.

Key words

Aldosterone Clock gene PAI-1 Cardiomyoblast 

Copyright information

© Springer-Verlag Tokyo 2007

Authors and Affiliations

  • Kyoe Tanaka
    • 1
  • Naoto Ashizawa
    • 1
  • Hiroaki Kawano
    • 1
  • Osami Sato
    • 1
  • Shinji Seto
    • 1
  • Eijun Nishihara
    • 2
  • Hideyuki Terazono
    • 2
  • Shojiro Isomoto
    • 1
  • Kazuyuki Shinohara
    • 2
  • Katsusuke Yano
    • 1
  1. 1.Division of Cardiovascular Medicine, Department of Translational Medical Sciences, Course of Medical and Dental SciencesGraduate School of Biomedical Sciences, Nagasaki UniversityNagasakiJapan
  2. 2.Division of Neurobiology and Behavior, Department of Basic Medical Sciences, Course of Medical and Dental SciencesGraduate School of Biomedical Sciences, Nagasaki UniversityNagasakiJapan