Biology and Fertility of Soils

, Volume 34, Issue 5, pp 349–356

Short-term changes in the spatial distribution of microorganisms in soil aggregates as affected by glucose addition

  •  C. Chenu
  •  J. Hassink
  •  J. Bloem
Original Paper

DOI: 10.1007/s003740100419

Cite this article as:
Chenu, C., Hassink, J. & Bloem, J. Biol Fertil Soils (2001) 34: 349. doi:10.1007/s003740100419

Abstract.

We studied the location of microorganisms in soil aggregates by low temperature scanning electron microscopy and by confocal laser microscopy (direct counts of bacteria) on dissected aggregates. We compared a sandy and a clayey soil and studied the effect of adding glucose on the spatial distribution of microorganisms. In the sandy soil, pores with diameters between 6 µm and 30 µm were the most abundant. In the clayey soil, the pores were <0.2 µm in diameter. In the sandy soil, microorganisms were present both on the surface and inside aggregates, whereas they were concentrated on the surface of clayey soil aggregates. Glucose addition increased the amount of bacteria and fungi on the surface of clayey soil aggregates but not inside them. For the sandy soil, microbial numbers were increased dramatically both inside and on the surface of the aggregates after glucose additions. This study thus evidenced contrasting microbial habitats in soil aggregates.

Soil aggregates Microorganisms Microhabitats Scanning electron microscopy 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  C. Chenu
    • 1
  •  J. Hassink
    • 2
  •  J. Bloem
    • 2
  1. 1.Unité de Science du Sol, INRA, 78026 Versailles Cedex, France
  2. 2.AB-DLO, DLO Institute for Agrobiology and Soil Fertility, P.O. Box 14, 67000 AA Wageningen, The Netherlands