Biology and Fertility of Soils

, Volume 33, Issue 6, pp 521–528

Degradation of isoproturon by the white rot fungus Phanerochaete chrysosporium

  • Maria del Pilar Castillo
  • Sabine von Wirén-Lehr
  • Irene Scheunert
  • Lennart Torstensson
Original Paper

DOI: 10.1007/s003740100372

Cite this article as:
del Pilar Castillo, M., von Wirén-Lehr, S., Scheunert, I. et al. Biol Fertil Soils (2001) 33: 521. doi:10.1007/s003740100372
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Abstract.

The ability of the white rot fungus Phanerochaete chrysosporium to degrade isoproturon was tested in solid substrate fermentation (SSF) cultures using straw as substrate/carrier material. The role of the lignin degrading enzymes, lignin peroxidase (LiP) and manganese peroxidase (MnP), in the degradation of the herbicide was also studied. Isoproturon concentration and LiP and MnP activities were followed in sterile straw cultures of the fungus. In vitro degradation tests with pure LiP and MnP were performed. P. chrysosporium in straw cultures was able to degrade 91% of the herbicide isoproturon in 14 days of incubation. A sharp decrease of isoproturon coincided with the largest MnP activity. Although LiP activity was also present, its role in SSF is unclear. The in vitro tests showed a strong isoproturon oxidation by LiP and a slower oxidation by MnP in the presence of Tween 80 probably by a lipid peroxidation process. Two N-demethylated metabolites were identified in pure enzyme tests and in SSF cultures. Several unidentified isoproturon derivatives, most likely hydroxylated, were also formed in both systems. The different pattern of derivatives detected in pure LiP and MnP tests showed a completely different metabolism by these two enzymes.

Isoproturon Biobeds Pesticides Degradation

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Maria del Pilar Castillo
    • 1
  • Sabine von Wirén-Lehr
    • 2
  • Irene Scheunert
    • 2
  • Lennart Torstensson
    • 1
  1. 1.Swedish University of Agricultural Sciences, Department of Microbiology, Box 7025, 750 07 Uppsala, Sweden
  2. 2.GSF-Research Centre for Environment and Health, Institute for Soil Ecology, Ingolstädter Landstrasse 1, 85764 Oberschleissheim, Germany
  3. 3.Present address: Vogtshaldenstrasse 47, 72074 Tübingen, Germany