Biology and Fertility of Soils

, Volume 31, Issue 1, pp 45–52

Variability of nodulation and dinitrogen fixation capacity among soybean cultivars

  • M. Hungria
  • T. R. J. Bohrer

DOI: 10.1007/s003740050622

Cite this article as:
Hungria, M. & Bohrer, T. Biol Fertil Soils (2000) 31: 45. doi:10.1007/s003740050622


 In order to identify soybean cultivars with higher biological N2 fixation capacities, North American and Brazilian soybean [Glycine max (L.) Merrill] cultivars, belonging to maturity groups VI–VIII, were evaluated for nodulation parameters and N2 fixation rates. The symbiotic performance of 152 cultivars was evaluated in pots containing 4 kg soil with an established population of the three Bradyrhizobium elkanii strains [29w (SEMIA 5019):SEMIA 566 : SEMIA 587, 22%:36%:34%] which are established in most Brazilian soils cultivated with soybean. Differences were verified among cultivars, with some accumulating up to twice as much nodule dry weight and N in tissues as others. The variability among cultivars was also confirmed when six of them were used in a field experiment, resulting in differences in nodulation, yield and total N accumulated in grains. The analysis of nodule occupancy in 12 cultivars grown either under sterile conditions and receiving a double inoculum and N-free nutrient solution, or in pots containing soil with an established population of bradyrhizobia, showed the preference of cultivars for specific strains.

Key words BradyrhizobiumGlycine maxNodulationSymbiosisPlant breeding

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Hungria
    • 1
  • T. R. J. Bohrer
    • 1
  1. 1.Embrapa-Soja, Cx. Postal 231, 86001-970, Londrina, PR, BrazilBR