Biology and Fertility of Soils

, Volume 29, Issue 1, pp 46–54

Arylsulfatase activity of microbial biomass in soils as affected by cropping systems

Authors

  • S. Klose
    • Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA e-mail: malit@iastate.edu, Fax: +1-515-2943163
  • J. M. Moore
    • Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA e-mail: malit@iastate.edu, Fax: +1-515-2943163
  • M. A. Tabatabai
    • Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA e-mail: malit@iastate.edu, Fax: +1-515-2943163
ORIGINAL PAPER

DOI: 10.1007/s003740050523

Cite this article as:
Klose, S., Moore, J. & Tabatabai, M. Biol Fertil Soils (1999) 29: 46. doi:10.1007/s003740050523

Abstract

 The impacts of crop rotations and N fertilization on different pools of arylsulfatase activity (total, intracellular, and extracellular) were studied in soils of two long-term field experiments in Iowa to assess the contibution of the microbial biomass to the activity of this enzyme. Surface-soil samples were taken in 1996 and 1997 in corn, soybeans, oats, or meadow (alfalfa) plots that received 0 or 180 kg N ha–1 before corn, and an annual application of 20 kg P ha–1 and 56 kg K ha–1. The arylsulfatase activity in the soils was assayed at optimal pH (acetate buffer, pH 5.8) before and after chloroform fumigation; microbial biomass C (Cmic) and N (Nmic) were determined by chloroform-fumigation methods. All pools of arylsulfatase activity in soils were significantly affected by crop rotation and plant cover at sampling time, but not by N fertilization. Generally, the highest total, intracellular, and extracellular arylsulfatase activities were obtained in soils under cereal-meadow rotations, taken under oats or meadow, and the lowest under continuous cropping systems.Total, intracellular, and extracellular arylsulfatase activities were significantly correlated with Cmic (r>0.41, P<0.01) and Nmic (r>0.38, P<0.01) in soils. The averages of specific activity values, i.e., of arylsulfatase activity of the microbial biomass, expressed per milligram Cmic, ranged from 315 to 407 μg p-nitrophenol h–1. The total arylsulfatase activity was significantly correlated with the intracellular activity, with r values >0.79 (P<0.001). In general, about 45% of the total arylsulfatase activity was extracellular, and 55% was associated with the microbial biomass in soils, indicating the importance of the microflora as an enzyme source in soils.

Key words Arylsulfatase activity Chloroform-fumigation method Biomass enzymes Crop rotations N fertilization

Copyright information

© Springer-Verlag Berlin Heidelberg 1999