Biology and Fertility of Soils

, Volume 26, Issue 2, pp 79–87

Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity

  • K. Y. Kim
  • D. Jordan
  • G. A. McDonald
ORIGINAL PAPER

DOI: 10.1007/s003740050347

Cite this article as:
Kim, K., Jordan, D. & McDonald, G. Biol Fertil Soils (1997) 26: 79. doi:10.1007/s003740050347

Abstract

The interaction of vesicular-arbuscular mycorrhizae (VAM) and phosphate-solubilizing bacteria (PSB) on plant growth, soil microbial activities, and the production of organic acids was studied in non-sterile soil containing hydroxyapatite and glucose. Glomus etunicatum (VAM), a fungus, and Enterobacter agglomerans, a bacterium able to solubilize insoluble phosphate, were used as inocula. Three treatments and a control were used: inoculation with E. agglomerans (treatment E), inoculation with G. etunicatum (treatment G), inoculation with E. agglomerans+G. etunicatum (treatment E+G) and the control (C). Inoculation with E, G, or E+G had increased plant growth by days 35, 55, and 75 compared with the control. Microbial biomass carbon (C) and alkaline phosphatase activity in the rhizosphere generally increased with time. Alkaline phosphatase activity was higher in treatments G and E+G compared with the control at 35 and 55 days. The highest acid phosphatase activity was observed in treatment E at 35 days; however, this markedly decreased with time. A significantly higher soluble phosphorus (P) concentration was observed in treatments E and E+G on day 55 compared with C. However, there was no significant difference in soluble P concentration in the rhizosphere between treatments with time. The P concentration was greatest in all treatments on day 55. The highest oxalic acid concentration was observed in the rhizosphere of the non-sterile soil in E+G on day 35. Total N and P uptake in plants from treatments E and G were higher compared with the control. However, the highest N and P uptake was observed in treatment E+G. This study suggests a synergistic interaction between E. agglomerans and G. etunicatum.

Key words Organic acidsPhosphate-solubilizingbacteriaVesicular-arbuscular mycorrhizaeGlomus etunicatumEnterobacter agglomerans

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • K. Y. Kim
    • 1
  • D. Jordan
    • 1
  • G. A. McDonald
    • 2
  1. 1.Department of Soil and Atmospheric Sciences, The School of Natural Resources, University of Missouri, Columbia, MO 65211, USAUS
  2. 2.Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, Columbia, MO 65211, USAUS
  3. 3.144 Mumford Hall, University of Missouri, Columbia, MO 65211, USA Tel.: 573/882-0090; Fax: 573/884-4960; e-mail: snrdiann@showme.missouri.eduUS