Biology and Fertility of Soils

, Volume 32, Issue 4, pp 318–320

Genetic variability in dinitrogen fixation between cowpea [Vigna unguiculata (L.) Walp] cultivars determined using the nitrogen-15 isotope dilution technique

  • M. A. F. Ndiaye
  • M. M. Spencer
  • M. Gueye
ORIGINAL PAPER

DOI: 10.1007/s003740000254

Cite this article as:
Ndiaye, M., Spencer, M. & Gueye, M. Biol Fertil Soils (2000) 32: 318. doi:10.1007/s003740000254

Abstract

 N fixed in 16 cultivars of cowpea [Vigna unguiculata (L.) Walp] inoculated with effective Bradyrhizobium strains collected from the West African MIRCEN culture collection was measured by 15N isotope dilution technique. In all plant parts, significant differences in the percentage of N derived from the atmosphere (%Ndfa) and the amount of Ndfa occurred between the cultivars. Ndoute variety exhibited the highest %Ndfa (74.33% in shoots; 60.90% in roots) and accumulated more fixed N (960 mg N plant–1 and 38 mg N plant–1 in shoots and roots, respectively). Therefore this cultivar should be selected as the highest N-fixing cowpea cultivar. It also should be used in a breeding programme to contribute to the development of cultivars that could stimulate an intensive use of cowpea in many different cropping systems in Africa with a view to maintaining soil fertility.

Key words Genetic variabilityVigna unguiculataNitrogen-15 methodNitrogen fixation

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. A. F. Ndiaye
    • 1
  • M. M. Spencer
    • 1
  • M. Gueye
    • 2
  1. 1.Université Cheikh A. Diop, Faculté des Sciences et Techniques, Département Biologie Végétale BP 5005, Dakar, SénégalSN
  2. 2.MIRCEN/Centre ISRA-IRD, BP 1386, Dakar, Sénégal e-mail: Mamadou.Gueye@ird.sn Tel.: +221-8493321 Fax: +221-8321675SN