Original Paper

Biology and Fertility of Soils

, 44:1073

First online:

Control of Fusarium wilt disease of cucumber plants with the application of a bioorganic fertilizer

  • Shusheng ZhangAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural UniversityJinhua College of Profession and Technology
  • , Waseem RazaAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Xingming YangAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Jiang HuAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Qiwei HuangAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Yangchun XuAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Xinghai LiuAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Wei RanAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University
  • , Qirong ShenAffiliated withCollege of Resources and Environmental Sciences, Nanjing Agricultural University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Two field experiments were conducted to evaluate the effect of organic fertilizer application either with or without antagonistic bacteria (Bacillus subtilis SQR-5 and Paenibacillus polymyxa SQR-21) on the control of Fusarium oxysporum f. sp. Cucumerinum J. H. Owen wilt disease in cucumber. The incidence of Fusarium wilt disease was 5.3–13.5% for cucumber plants treated with bioorganic fertilizer, while it was 30.3–51% in controls (only with organic fertilizer). Higher yields and lower disease incidences were observed in the dry season when compared with the wet season for both types of organic fertilizer treatments. Biolog analysis showed a significant change in soil bacterial composition and activity after bioorganic fertilizer application. The numbers of colony-forming units of F. oxysporum f. sp. Cucumerinum J. H. Owen for bioorganic-fertilizer-treated soils were significantly decreased compared with control. Scanning electron micrographs of cucumber basal stems showed a presence of mycelia-like mini strands accompanied by an amorphous substance within the xylem vessels. This amorphous substance and mini strands were richer in calcium and phosphorus but had low carbon and oxygen than the living mycelia. Reverse-phase high-pressure liquid chromatography and mass spectroscopic analysis showed that the antagonistic bacteria produced the antifungal compounds fusaricidin A, B, C, and D with molecular weights of 883.5, 897.5, 947.5, and 961.5 Da, respectively. The application of bioorganic fertilizer has a great potential for the control of F. oxysporum wilt disease in cucumber plants.

Keywords

Antagonistic substance Bacillus subtilis Bioorganic fertilizer Cucumber plant Fusarium wilt Paenibacillus polymyxa