Biology and Fertility of Soils

, Volume 43, Issue 2, pp 137–143

Infection and colonization of aseptically micropropagated sugarcane seedlings by nitrogen-fixing endophytic bacterium, Herbaspirillum sp. B501gfp1

  • Joyce Njoloma
  • Kazunori Tanaka
  • Tasuku Shimizu
  • Tomohiro Nishiguchi
  • Muhammad Zakria
  • Ryo Akashi
  • Moriya Oota
  • Shoichiro Akao
Original Paper

DOI: 10.1007/s00374-006-0078-5

Cite this article as:
Njoloma, J., Tanaka, K., Shimizu, T. et al. Biol Fertil Soils (2006) 43: 137. doi:10.1007/s00374-006-0078-5

Abstract

In this study, we used Herbaspirillum sp. B501gfp1 (B501gfp1), an isolate from wild rice, to investigate the interaction between a non-host nitrogen-fixing endophytic bacterium and micropropagated sugarcane plants under aseptic condition. Two Japanese sugarcane plants (Saccharum sp.) cultivars (cvs) NiF8 and Ni15 were inoculated using B501gfp1 in two inoculum doses of 108 and 102 bacterial-cells-per-milliliter suspension. The results showed that bacterial cells colonized both the root and stem tissues, and colonization was apparent in the intercellular spaces. Higher bacterial numbers were detected in plant tissues inoculated with the higher inoculum concentration treatment. Bacterial numbers also varied between the two cultivars, with the higher values determined in cv Ni15. This study provides evidence that Herbaspirillum sp. B501gfp1, a rice isolate, could colonize sugarcane tissues, suggesting non-specificity of host plant among endophytes.

Keywords

Herbaspirillum sp. B501gfp1Sterile sugarcane plantsInoculum concentrationBacterial numbersEndophytic colonization

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Joyce Njoloma
    • 1
  • Kazunori Tanaka
    • 1
  • Tasuku Shimizu
    • 1
  • Tomohiro Nishiguchi
    • 1
  • Muhammad Zakria
    • 1
  • Ryo Akashi
    • 1
  • Moriya Oota
    • 2
  • Shoichiro Akao
    • 1
  1. 1.Faculty of AgricultureMiyazaki UniversityMiyazaki,Japan
  2. 2.Miyako branchOkinawa Prefectural Agricultural Experiment StationHirara,Japan