Journal of Comparative Physiology B

, Volume 180, Issue 7, pp 1005–1018

Metabolic and molecular stress responses of gilthead seam bream Sparus aurata during exposure to low ambient temperature: an analysis of mechanisms underlying the winter syndrome

Authors

  • Themis-Dimitrios Kyprianou
    • Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of BiologyAristotle University of Thessaloniki
  • Hans O. Pörtner
    • Alfred-Wegener-Institut für Polar-und MeeresforschungPhysiologie mariner Tiere
  • Andreas Anestis
    • Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of BiologyAristotle University of Thessaloniki
  • Basile Kostoglou
    • Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of BiologyAristotle University of Thessaloniki
  • Konstantinos Feidantsis
    • Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of BiologyAristotle University of Thessaloniki
    • Laboratory of Animal Physiology, Department of Zoology, Faculty of Sciences, School of BiologyAristotle University of Thessaloniki
Original Paper

DOI: 10.1007/s00360-010-0481-y

Cite this article as:
Kyprianou, T., Pörtner, H.O., Anestis, A. et al. J Comp Physiol B (2010) 180: 1005. doi:10.1007/s00360-010-0481-y

Abstract

The winter syndrome in the gilthead sea bream Sparus aurata indicates that the species is exposed to critically low temperatures in Mediterranean aquaculture in winter. The present study of metabolic patterns and molecular stress responses during cold exposure was carried out to investigate this “disease”, in light of the recent concept of oxygen and capacity limited thermal tolerance. The metabolic profile of fuel oxidation was examined by determining the activities of the enzymes hexokinase (HK), aldolase (Ald), pyruvate kinase (PK), l-lactate dehydrogenase (l-LDH), citrate synthase (CS), malate dehydrogenase (MDH) and 3-hydroxyacyl CoA dehydrogenase (HOAD) in heart, red and white muscle after exposure to temperatures of 10, 14 and 18°C. Especially, the increase in LDH activity combined with the accumulation of l-lactate in tissues indicates that temperatures below 14°C are critical for Sparus aurata and stimulate the anaerobic component of metabolism. Increase in the activity of HOAD suggests that oxidation of free fatty acids might contribute to ATP turnover at low temperatures. The expression of Hsp70 and Hsp90 in all tissues examined revealed a cellular stress response during cooling below 18°C. In the light of winter temperatures in S. aurata cultures around 10°C, our data suggest that the fish are exposed to stressful conditions at the low end of their thermal tolerance window. These conditions likely impair the aerobic capacity of the fish, compromise the rates of growth and reproduction and may contribute to elicit pathological conditions.

Keywords

Low temperatureMarine fishMetabolic adjustmentsHsps

Copyright information

© Springer-Verlag 2010