Journal of Comparative Physiology B

, Volume 180, Issue 4, pp 591–598

Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine

  • Alexander G. Markov
  • Anna Veshnyakova
  • Michael Fromm
  • Maren Amasheh
  • Salah Amasheh
Original Paper

DOI: 10.1007/s00360-009-0440-7

Cite this article as:
Markov, A.G., Veshnyakova, A., Fromm, M. et al. J Comp Physiol B (2010) 180: 591. doi:10.1007/s00360-009-0440-7

Abstract

In tubular epithelia, barrier function varies in a segment-specific way. The aim of this study was to correlate the presence of tight junction proteins and paracellular barrier properties along rat intestine. Tissue segments of duodenum, jejunum, ileum, and colon were stripped of submucosal cell layers and mounted in Ussing chambers for impedance spectroscopy to measure epithelial resistance (Repi). In parallel, expression of tight junction proteins was analysed by Western blots and immune fluorescence confocal microscopy. Colon showed highest Repi, followed by duodenum, jejunum, and ileum. In small intestine, common transepithelial resistance (Rtrans or TER) overestimated true Repi by ~60%. In colon, strongest expression of “tightening” claudins 1, 3, 4, 5, and 8 was detected. In accordance with Repi the most proximal of the small intestinal segments, duodenum exhibited highest expression of “tightening” claudins and lowest expression of claudins mediating permeability, namely claudin-2, -7, and -12, compared to jejunum and ileum. These results correspond to the specific role of the duodenum as the first segment facing the acidic gastric content.

Keywords

Intestine Barrier Tight junctions Claudins 

Abbreviations

TER

Transepithelial Resistance

TJ

Tight Junction

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Alexander G. Markov
    • 1
  • Anna Veshnyakova
    • 2
  • Michael Fromm
    • 3
  • Maren Amasheh
    • 4
  • Salah Amasheh
    • 3
  1. 1.Biological and Soil Faculty of St. Petersburg UniversitySt. PetersburgRussia
  2. 2.Leibniz Institute of Molecular Pharmacology (FMP)BerlinGermany
  3. 3.Institute of Clinical Physiology, Charité Campus Benjamin FranklinBerlinGermany
  4. 4.Department of GastroenterologyCharité, Campus Benjamin FranklinBerlinGermany

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