Journal of Comparative Physiology B

, Volume 173, Issue 8, pp 695–702

High content of polyunsaturated fatty acids in muscle phospholipids of a fast runner, the European brown hare (Lepus europaeus)

Original Paper

DOI: 10.1007/s00360-003-0382-4

Cite this article as:
Valencak, T.G., Arnold, W., Tataruch, F. et al. J Comp Physiol B (2003) 173: 695. doi:10.1007/s00360-003-0382-4

Abstract

To investigate both seasonal changes and possible intracorporal gradients of phospholipid fatty acid composition, skeletal muscles (n=124), hearts (n=27), and livers (n=34) from free-living brown hares (Lepus europaeus) were analyzed. Phospholipids from both skeletal muscles and heart had a high degree of unsaturation with 66.8±0.63% and 65.7±0.5% polyunsaturated fatty acids, respectively. This is the highest proportion of polyunsaturated fatty acids reported in any mammalian tissue. Polyunsaturated fatty acid content in skeletal muscles was 2.3% greater in winter compared to summer (F1,106=17.7; P=0.0001), which may reflect thermoregulatory adjustments. Arachidonate (C20:4n-6) showed the greatest seasonal increase (+2.5%; F=7.95; P=0.0057). However, there were no pronounced differences in polyunsaturated fatty acid content between skeletal muscles from different locations in the body (m. iliopsoas, m. longissimus dorsi and m. vastus). Total muscle phospholipid polyunsaturated fatty acid content was correlated with polyunsaturated fatty acid content in triacyglycerols from perirenal white adipose tissue depots (r2=0.61; P=0.004). Polyunsaturated fatty acids were enriched in muscle phospholipids (56.8–73.6%), compared to white adipose tissue lipids (20.9–61.2%), and liver phospholipids (25.1–54.2%). We suggest that the high degree of muscle membrane unsaturation is related to hare-specific traits, such as a high maximum running speed.

Keywords

Fatty acid compositionLepus europaeusMembrane unsaturationMuscle phospholipidsSeason

Abbreviations

BMR

basal metabolic rate

DPA

docosapentaenoic acid

DHA

docosahexaenoic acid

FA

fatty acid

MUFA

monounsaturated fatty acid

PC

principal component

PUFA

polyunsaturated fatty acid

SFA

saturated fatty acid

UI

unsaturation index

WAT

white adipose tissue

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • T. G. Valencak
    • 1
  • W. Arnold
    • 1
  • F. Tataruch
    • 1
  • T. Ruf
    • 1
  1. 1.Research Institute of Wildlife EcologyUniversity of Veterinary MedicineViennaAustria