World Journal of Urology

, Volume 32, Issue 5, pp 1199–1204

Is real-time PCR the correct method to evaluate the incidence of human papillomavirus in prepuces of asymptomatic boys and men?

Authors

  • Isabel Heidegger
    • Department of UrologyMedical University of Innsbruck
  • Renate Pichler
    • Department of UrologyMedical University of Innsbruck
  • Barbara Müller
    • Department of UrologyMedical University of Innsbruck
  • Helmut Klocker
    • Department of UrologyMedical University of Innsbruck
  • David Oswald
    • Medical University of Salzburg
  • Bernhard Haid
    • Department of Paediatric UrologyHospital St Vinzenz
  • Bettina Zelger
    • Department of UrologyMedical University of Innsbruck
  • Wolfgang Horninger
    • Department of UrologyMedical University of Innsbruck
    • Department of Paediatric UrologyHospital St Vinzenz
    • Department of UrologyMedical University of Innsbruck
Original Article

DOI: 10.1007/s00345-013-1190-4

Cite this article as:
Heidegger, I., Pichler, R., Müller, B. et al. World J Urol (2014) 32: 1199. doi:10.1007/s00345-013-1190-4

Abstract

Objective

To investigate the prevalence of human papillomavirus (HPV) in prepuces of asymptomatic boys and men, the present study was designed.

Methods

Two hundred and fifty male prepuce specimens who underwent circumcision due to phimosis were collected. Samples were subdivided into groups regarding their age: children (group I, 0–10 years), adolescents (group II, 11–20 years) and adults (group III, >20 years). HPV High Screen Real-TM Quant 2x kit detecting HPV 6 and 11 (low risk) as well as another kit for identification of HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58 and 59 (high risk) were used. Additionally, a Taq Man assay has been designed targeting the L1 gene of HPV 6, 11, 16 and 18.

Results

Evaluating the number of low-risk HPV subtypes, we found HPV 6 and 11 in 5.3 % of samples (n = 12/226). Concerning high-risk HPV, we found a positivity in 4 % of samples (n = 9/224). In contrast to low-risk data where no age distribution was observed, we found an age-specific accumulation of high-risk HPV subtypes in the children group (n = 6/9). A second independent assay (Taq Man PCR assay) measuring HPV 6, 11, 16 and 18 of all positive samples confirmed only the high-risk HPV subtypes of the Real-TM Quant 2x assay.

Conclusions

Our study provides evidence that qPCR estimation for HPV infection obviously underestimates the incidence rate of infected prepuces in boys and men with phimosis. Contrary, an overestimation of the HPV infection rate with the in situ hybridization method of phimotic prepuces cannot be excluded.

Keywords

Low-risk HPVHigh-risk HPVPrepucePhimosisReal-time PCR

Abbreviations

HPV

Human papilloma virus

DNA

Deribonuclein acid

ISH

In situ hybridization

qPCR

Real-time polymerase change reaction

Copyright information

© Springer-Verlag Berlin Heidelberg 2013