Journal of Plant Growth Regulation

, Volume 32, Issue 4, pp 758–766

Characterization of IAA Production by the Mangrove Cyanobacterium Phormidium sp. MI405019 and Its Influence on Tobacco Seed Germination and Organogenesis

  • Thangavelu Boopathi
  • Vadivel Balamurugan
  • Selvaraj Gopinath
  • Muthuraman Sundararaman
Article

DOI: 10.1007/s00344-013-9342-8

Cite this article as:
Boopathi, T., Balamurugan, V., Gopinath, S. et al. J Plant Growth Regul (2013) 32: 758. doi:10.1007/s00344-013-9342-8

Abstract

The production of indole-3-acetic acid (IAA) by a mangrove root-associated cyanobacterium, Phormidium sp. MI405019, was demonstrated in this study. The extracellular extract (ECE) of this cyanobacterial culture filtrate was tested on tobacco seed germination and callus differentiation. In ECE treatment, seed germination was increased by 40 % when compared to that of control. In addition, ECE also induced multiple roots from tobacco callus. Further, the factors such as concentration of l-tryptophan and NaCl (salinity) on IAA production were studied. IAA in the medium was initially increased up to a certain period and subsequently decreased in all salinity ranges tested. The amount of IAA production was decreased after 48 h in the culture grown in media amended with 2 % NaCl and without NaCl. However, the IAA concentration was increased up to 96 h in media containing 4 % NaCl. IAA produced by Phormidium sp. MI405019 was extracted from the culture filtrate and its identity was confirmed by thin-layer chromatography and ultra-fast liquid chromatography. To the best of our knowledge, this is the first report of IAA production by cyanobacteria isolated from a mangrove ecosystem. Based on the results in this study, the utilization of Phormidium sp. MI405019 in the aspects of mangrove growth promotion was discussed.

Keywords

Indole-3-acetic acidMangrove cyanobacteriaSalinityBioactivityPlant growth promotion16S rDNA

Supplementary material

344_2013_9342_MOESM1_ESM.docx (4.9 mb)
Supplementary material 1 (DOCX 5032 kb)

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Thangavelu Boopathi
    • 1
  • Vadivel Balamurugan
    • 1
  • Selvaraj Gopinath
    • 1
  • Muthuraman Sundararaman
    • 1
  1. 1.Department of Marine Biotechnology, National Facility for Marine CyanobacteriaBharathidasan UniversityTiruchirappalliIndia